Production of peptides and proteins by accumulation in plant endoplasmic reticulum-derived protein bodies
AssigneeEra Biotech, S.A.
InventorsMaria Dolores Ludevid Múgica, Margarita Torrent Quetglas, Sabine Ramassamy
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Abstract
A nucleic acid molecule is disclosed as containing a first nucleic acid sequence comprising a nucleotide sequence that encodes a γ-zein protein, or a fragment thereof, that is capable of directing and retaining a protein towards the endoplasmic reticulum (ER) of a plant cell and a nucleic acid sequence encoding a peptide or protein of interest. Methods of using this nucleic acid molecule for transforming host plant cells in order to improve the production and purification of the peptide or protein of interest are also disclosed. Polypeptides encoded by the nucleic acids and produced the methods are also disclosed.
Claims7
A fusion protein comprising:
- (i) a fragment of γ-zein that comprises amino acids 20 to 113 of SEQ ID NO:4, and (ii) a protein of interest.
Dependencies are inferred from the claim text.