Microchips, kits, and methods for immunoassays

An immunoassay microchip, comprising a microchannel(s) comprising a flow path(s) with a microstructure(s) having a three dimensional structure,

• wherein the microstructure(s) retains microbeads uniformly dispersed in a photo-cured hydrophilic resin in all directions of the three dimensional structure;
• wherein the microbeads have a primary antibody immobilized on their surface;
• and
• wherein the microstructure(s) is patterned into an arbitrary shape by light exposure treatment of the hydrophilic light-curable resin solution in which the microbeads are suspended;
• wherein the microstructure(s) has an external diameter of about 40 μm (in width) and a height of 100 μm (in depth);
• and
• wherein a cross-section of the microchannel is divided into a flow path portion and a microstructure portion retaining uniformly-dispersed beads so that the flow path portion in the microchannel is secured between the microstructures without large increase in flow path resistance.