Extracellular matrix coated surface for culturing cells
AssigneeCorning Incorporated
InventorsSusan X. Qian, Suparna Sanyal
Life of this patent
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Abstract
A cell culture product is provided for propagating embryonic stem cells, and maintaining their self-renewal and pluripotency characteristics for extended periods of time in culture. The cell culturing product includes a substrate; and a coating thereon deposited from a coating solution. The coating solution includes a mixture of extracellular matrix proteins and an aqueous solvent, wherein the total protein concentration in the coating solution is about 10 μg/ml to about 1 mg/ml.
Claims14
A cell culture product comprising:
- a substrate;
- and
- a coating thereon;
- wherein the coating is produced by a method comprising:
- applying a coating solution to the substrate, wherein the coating solution comprises a mixture of extracellular matrix proteins including laminin and at least one other extracellular matrix component and an aqueous solvent, wherein the total protein concentration in the coating solution is about 10 μg/ml to about 1 mg/ml;
- incubating the coating solution on the substrate;
- and then
- washing the substrate to remove unbound extracellular matrix proteins.
A cell culture product comprising:
- a substrate;
- and
- an extracellular matrix coating thereon, wherein the coating is provided to at least one surface of the substrate in a solution at a minimal concentration sufficient to saturate the substrate surface with extracellular matrix proteins;
- and then the substrate is washed to remove unbound extracellular matrix proteins.
An apparatus for propagating pluripotent embryonic stem cells comprising:
- a) a container suitable for culturing cells;
- and
- b) an extracellular matrix in a fixed concentration on at least one surface of the container;
- wherein said extracellular matrix comprises a mixture of laminin and at least one other extracellular matrix component, and
- wherein the extracellular matrix is provided to the at least one surface by coating at least one surface of the container with the extracellular matrix, washing the surface coated with extracellular matrix to remove unbound extracellular matrix proteins, and then drying the container such that the extracellular matrix is in a dried state on at least one surface of said container.
Dependencies are inferred from the claim text.