Construction of a quadruple enterotoxin-deficient mutant of Bacillus thuringiensis
AssigneeWisconsin Alumni Research Foundation
InventorsJo Emily Handelsman, Amy Klimowicz, Changhui Guan
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Abstract
Some HBL and NHE enterotoxins are known to cause food-borne diseases in humans. Enterotoxin-deficient mutants of member strains of the Bacillus cereus group that do not produce HBL, HBLa1, HBLa2, or NHE enterotoxins are disclosed. Enterotoxin-deficient mutants are suitable for use as biocontrol agents. Methods for making the mutants and for using the mutants are described.
Claims4
An isolated Bacillus thuringiensis strain comprising a disabling mutation at locus nhe whereby NHE enterotoxin is not produced, a disabling mutation at locus hbla2 whereby enterotoxin HBLa2 is not produced, and a disabling mutation at one or more of loci hbl and hbla1, whereby at least one of enterotoxins HBL and HBLa1 is not produced.
Dependencies are inferred from the claim text.