Production of viruses, viral isolates and vaccines
AssigneeCrucell Holland B.V.
InventorsGiuseppe Marzio, Maria Grazia Pau, Dirk Jan Elbertus Opstelten, Alphonsus Gerardus Cornelis Maria Uytdehaag
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Abstract
The present invention discloses methods for producing and/or propagating virus particles, such as influenza virus particles, that are present in a virus isolate obtained from an infected subject by contacting a host cell with a virus particle and culturing the cell under conditions conducive to propagation of the virus particle. The invention also provides a method for selective propagation of a set of virus particles, such as influenza virus particles present in an influenza isolate, which have an affinity for receptors comprising a specific glycosylation residue.
Claims14
A method for producing an influenza virus particle, said method comprising the steps of:
- contacting a cell that is able to propagate influenza virus particles with an influenza virus particle in a culture medium under conditions conducive to infection of said cell by said influenza virus particle, wherein the influenza virus particle is from an influenza isolate obtained from a subject infected with influenza virus;
- and culturing said cell under conditions conducive to propagation of said influenza virus particle, wherein said cell over-expresses a heterologous nucleic acid encoding an alpha2,6 sialyltransferase or a functional equivalent thereof as compared to said cell that does not comprise the heterologous nucleic acid.
A method for producing an influenza virus particle, the method comprising:
- providing a host cell that is able to propagate influenza virus particles;
- over expressing a heterologous nucleic acid encoding alpha2,3 sialyltransferase in the host cell as compared to said cell that does not comprise the heterologous nucleic acid;
- infecting the host cell with an influenza virus particle, wherein the influenza virus particle is from an influenza isolate obtained from a subject infected with influenza virus;
- and
- culturing the host cell under conditions which allow propagation of the influenza virus particle.
A method for producing an influenza virus particle, the method comprising:
- providing a PER.C6 host cell;
- over expressing a heterologous nucleic acid encoding alpha2,3 sialyltransferase in the host cell as compared to said cell that does not comprise the heterologous nucleic acid;
- infecting the host cell with an influenza virus particle, wherein the influenza virus particle is from an influenza isolate obtained from a subject infected with influenza virus;
- and
- culturing the host cell under conditions which allow propagation of the influenza virus particle.
A method of producing influenza virus, the method comprising:
- growing a clinical isolate of influenza in a PER.C6 cell expressing a heterologous alpha 2,6, sialotransferase.
Dependencies are inferred from the claim text.