Talaromyces transformants
AssigneeDsm IP Assets B.V.
InventorsAlrik Pieter Los, Brenda Vonk, Marco Alexander Van Den Berg, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt, Adrianus Wilhelmus Hermanus Vollebregt, Margot Elisabeth Francoise Schooneveld-Bergmans
Life of this patent
Estimating the term…
Abstract
The invention relates to a Talaromyces transformant comprising one or more recombinant gene, capable of producing cellulase in the absence of cellulase inducer in a glucose medium, having a cellulase activity of 2 WSU/ml or more, in 16 times or more diluted supernatant or broth.
Claims17
A process for production of a Talaromyces emersonii transformant, said process comprising the steps of:
- (a) providing one or more expression cassettes comprising at least one polynucleotide encoding cellulose, hemicellulase, and/or pectinase, and at least one promoter for expression of said polynucleotide;
- (b) providing a selection marker included in the expression cassette of (a) and/or included in a dedicated selection marker polynucleotide;
- (c) transfecting a Talaromyces emersonii host which endogenously produces cellulase and/or hemicellulase with the at least one expression cassette from (a) and the selection marker from (b);
- (d) selecting the Talaromyces emersonii transformant which comprises said at least one expression cassette from (a) and the selection marker from (b);
- and
- (e) isolating said Talaromyces emersonii transformant.
A process for production of a polypeptide composition comprising at least one cellulase, hemicellulase, and/or pectinase comprising the steps of:
- (a) providing one or more expression cassettes comprising at least one polynucleotide encoding cellulase, hemicellulase, and/or pectinase, and at least one promoter for expression of said polynucleotide;
- (b) providing a selection marker included in the expression cassette of (a) and/or included in a dedicated selection marker polynucleotide;
- (c) transfecting a Talaromyces emersonii host with the at least one expression cassette from (a) and/or the selection marker from (b);
- (d) selecting the Talaromyces emersonii transformant which comprises said at least one expression cassette from (a) and the selection marker from (b);
- and
- (e) producing the polypeptide composition comprising cellulase, hemicellulase, and/or pectinase by culturing the Talaromyces emersonii transformant in a suitable culture medium in which a cellulase inducer is substantially absent;
- and
- (f) recovering the polypeptide composition comprising cellulase, hemicellulase, and/or pectinase.
Dependencies are inferred from the claim text.