Polynucleotides having promoter activity
AssigneeNovozymes A/S
InventorsHiroshi Teramoto, Hiroaki Udagawa, Rikako Taira
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Abstract
The present invention relates to isolated polynucleotides having promoter activity the use of the isolated polynucleotides for the production of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.
Claims12
A nucleic acid construct comprising an isolated polynucleotide having promoter activity operably linked to a heterologous coding sequence encoding a polypeptide, wherein the polynucleotide having promoter activity is selected from the group consisting of:
- (a) a polynucleotide comprising a nucleotide sequence having promoter activity having at least 95% sequence identity to the nucleotide sequence of SEQ ID NO:
- 1;
- and (b) a polynucleotide having promoter activity that hybridizes with the nucleotide sequence of SEQ ID NO:
- 1, or the full-length complement thereof, under stringency conditions defined as hybridization at 42° C. in DIG Easy Hyb buffer and washing twice in 2X SSC at room temperature for 5 minutes and twice in 0.1X SSC at 68° C. for 15 minutes each.
Dependencies are inferred from the claim text.