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US 8,802,411 B2Granted
ADP detection based luminescent phosphotransferase or ATP hydrolase assay
AssigneePromega Corporation
InventorsHicham Zegzouti, Said A. Goueli
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Abstract
The invention provides compositions and methods to determine or detect the activity of enzymes, including phosphotransferases such as kinases (e.g., protein, lipid, and sugar kinases) and ATP hydrolases such as ATPases, e.g., HSP90, that employ ATP as a substrate and form ADP as a product by monitoring changes in ADP. Methods, kits and compositions for monitoring changes in metabolites such as ADP are disclosed.
Claims11
A kit for detecting ADP in a solution comprising:
- (i) an adenylate cyclase;
- (ii) an enzyme that is capable of catalyzing the conversion of ADP to ATP;
- (iii) a luciferase enzyme;
- and
- (iv) a substrate for the luciferase enzyme.
2 more elements
Dependencies are inferred from the claim text.