Mutant polyhydroxyalkanoic acid synthase gene and method for producing aliphatic polyester using the same
AssigneeToyota Jidosha Kabushiki Kaisha
InventorsMasayoshi Muramatsu, Hiromi Kambe, Masakazu Ito
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Abstract
A substitution mutation that improves polymerization activity of a polyhydroxyalkanoic acid synthase is identified. At least 1 amino acid residue selected from the group consisting of a histidine residue at position 17, a proline residue at position 71, a valine residue at position 131, a methionine residue at position 205, a leucine residue at position 230, and a proline residue at position 239 of a polyhydroxyalkanoic acid synthase derived from Alcanivorax borkumensis is subjected to substitution mutation with another amino acid.
Claims15
A nucleic acid encoding a polypeptide having an amino sequence of SEQ ID NO:2, wherein the proline residue at position 239 is substituted with leucine.
A nucleic acid encoding a polypeptide having an amino sequence of SEQ ID NO:2, wherein the valine residue at position 131 is substituted with isoleucine.
A nucleic acid encoding a polypeptide having an amino sequence of SEQ ID NO:2, wherein the histidine residue at position 17 is substituted with leucine, the proline residue at position 71 is substituted with serine, and the methionine residue at position 205 is substituted with threonine.
A nucleic acid encoding a polypeptide having an amino sequence of SEQ ID NO:2, wherein the leucine residue at position 230 is substituted with methionine.
Dependencies are inferred from the claim text.