Methods and compositions for preparation of selectively protected carbohydrates
AssigneesCalifornia Institute of Technology, The Scripps Research Institute
InventorsFrances H. Arnold, Chi-Huey Wong, Yuuichi Mitsuda, Michael M. Chen, Clay Bennett, William Greenberg, Jared Crawford Lewis, Sabine Bastian
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Abstract
The disclosure relates to engineered P450 polypeptides and use of such polypeptides in chemoenzymatic methods to construct selectively protected carbohydrates, which are useful as building blocks for preparation of carbohydrate derivatives and oligosaccharides
Claims14
A method for regioselective removal of alkyl ethers groups on protected peralkylated carbohydrates comprising contacting the carbohydrate with a polypeptide having at least 95% identity to a polypeptide comprising a sequence selected from the group consisting of SEQ ID NO:1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 and 13, wherein the polvpeptide has monooxygenase activity, and wherein the alkyl ether is removed.
Dependencies are inferred from the claim text.