Method for measuring the migration of cells in a channel under the influence of an analyte
AssigneeCellix Limited
InventorsDmitry Kashanin, Igor Shvets, Vivienne Williams, Frank O'Dowd
Life of this patent
Estimating the term…
Abstract
The present invention is directed to a method for measuring the migration of cells in a channel under the influence of an analyte wherein said cells are separated from said analyte by a semi-permeable membrane and said cells are subjected to controlled flow conditions while the analyte is static and in the form of a viscous substance or gel.
Claims18
A method for measuring the transmigration of cells in an assembly comprising a sample channel and an analyte channel or chamber which are in fluid communication in an overlap region between the sample channel and the analyte channel or chamber, said sample channel being separated from said analyte channel or chamber in said overlap region by a semi-permeable membrane mounted between the sample channel and the analyte channel or chamber, the method including:
- introducing a reagent into the analyte channel or chamber mixed with a gel which is a solid gel or is initially in the form of a liquid that is allowed to solidify in the analyte channel or chamber,
- delivering sample cells in a liquid carrier medium at a controlled flow rate through the sample channel on one side of the semi-permeable membrane whereby said sample cells are separated from the reagent in the analyte channel or chamber by the semi-permeable membrane, and
- measuring the transmigration of sample cells from the sample channel through the semi-permeable membrane into the analyte channel or chamber under the influence of the reagent in the analyte channel or chamber.
A method for measuring the transmigration of cells in an assembly comprising a sample channel and an analyte channel or chamber which are in fluid communication in an overlap region between the sample channel and the analyte channel or chamber, said sample channel being separated from said analyte channel or chamber in said overlap region by a semi-permeable membrane mounted between the sample channel and the analyte channel or chamber, the method including:
- introducing a reagent into the analyte channel or chamber mixed with a gel which is a solid gel or is initially in the form of a liquid that is allowed to solidify in the analyte channel or chamber,
- coating at least one side of the semi-permeable membrane with a substance which promotes adhesion of cells to the semi-permeable membrane,
- forming a layer of seeded cells on the semi-permeable membrane,
- delivering sample cells in a liquid carrier medium at a controlled flow rate through the sample channel on one side of the semi-permeable membrane whereby said sample cells are separated from the reagent in the analyte channel or chamber by the semi-permeable membrane, and
- measuring the transmigration of sample cells from the sample channel through the layer of seeded cells under the influence of the reagent in the analyte channel or chamber.
Dependencies are inferred from the claim text.