Method for testing or screening protein synthesis inhibitors
AssigneeThe Trustees of the University of Pennsylvania
InventorsGideon Dreyfuss, Mumtaz Kasim
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Abstract
This invention provides methods for screening an inhibitor of protein synthesis by measuring the level of relocalization of an SMN complex component from the cytoplasm to the nucleus. Additionally, the invention provides a kit and a system for screening protein synthesis inhibitors in a cell.
Claims16
A method for testing a candidate compound for an ability to inhibit protein synthesis in a cell, comprising the steps of:
- contacting said cell with said candidate compound;
- and
- measuring a level of an SMN complex component in the nucleus of said cell, whereby, if said candidate compound increases the amount of the SMN complex component in said nucleus then said compound exhibits an ability to inhibit protein synthesis, wherein said cell is a eukaryotic cell.
A method for testing a candidate compound for an ability to inhibit protein synthesis in a cell, comprising the steps of:
- contacting said cell with said candidate compound;
- and
- measuring a level of an SMN complex component in the nucleus of said cell, whereby, if said candidate compound increases the amount of the SMN complex component in said nucleus then said compound exhibits an ability to inhibit protein, wherein said method further comprises the step of contacting said cell with a ligand selective for a SMN complex component.
A method for testing a candidate compound for an ability to inhibit protein synthesis in a cell, comprising the steps of:
- contacting said cell with said candidate compound;
- and
- measuring a level of an SMN complex component in the nucleus of said cell, whereby, if said candidate compound increases the amount of the SMN complex component in said nucleus then said compound exhibits an ability to inhibit protein synthesis, wherein said SMN complex component is fused to a fluorescent probe.
A method for testing a candidate compound for an ability to inhibit protein synthesis in a cell, comprising the steps of:
- contacting said cell with said candidate compound;
- and
- measuring a level of an SMN complex component in the nucleus of said cell, whereby, if said candidate compound increases the amount of the SMN complex component in said nucleus then said compound exhibits an ability to inhibit protein synthesis, wherein said SMN complex component is a Gem.
A method for testing a candidate compound for an ability to inhibit protein synthesis in a cell, comprising the steps of:
- contacting said cell with said candidate compound;
- and
- measuring a level of an SMN complex component in the nucleus of said cell, whereby, if said candidate compound increases the amount of the SMN complex component in said nucleus then said compound exhibits an ability to inhibit protein synthesis, wherein said SMN complex component is a Gemin.
A method for testing a candidate compound for an ability to inhibit protein synthesis in a cell, comprising the steps of:
- contacting said cell with said candidate compound;
- and
- measuring a level of an SMN complex component in the nucleus of said cell, whereby, if said candidate compound increases the amount of the SMN complex component in said nucleus then said compound exhibits an ability to inhibit protein synthesis, wherein the step of measuring comprises the use of visualization means to identify said SMN complex components.
Dependencies are inferred from the claim text.