Potency test for vaccine formulations
AssigneesIntervet Inc., Intervet International B.V.
InventorsMichelle Allen, Mark Garrett, Urs Peter Bruderer, Martinus Antonius Johannes Thijssen
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Abstract
The invention relates to certain methods for the determination of an antigen content of a first antigen in a mixture comprising two or more antigens. The invention also relates to a potency test for an antigen in a combination vaccine. The method allows the determination of the antigen content in a mixture additionally comprising antibodies that are capable of binding with the antigen.
Claims19
A method for the determination of an antigen content of a porcine circovirus type 2 (PCV-2) antigen in a mixture of at least a first composition comprising the PCV-2 antigen and a second composition comprising (i) a Mycoplasma hyopneumoniae (M. hyo) antigen and (ii) antibodies directed against the PCV-2 antigen that are capable of binding with the PCV-2 antigen, the method comprising the steps of,
- a) mixing together the first and second compositions,
- b) dissociating antigen-antibody complexes in a sample of the mixture, formed between the PCV-2 antigen and the antibodies, and
- c) determining the antigen content of the PCV-2 antigen by means of an immunoassay.
Dependencies are inferred from the claim text.