Methods for quantitative cDNA analysis in single-cell

A method of detecting a nucleic acid, said method comprising in sequence:

• picking up a whole single-cell from a sample containing at least a single-cell having a cell membrane,
• lysing the cell membrane of the sampled whole single-cell and extracting nucleic acids from the cell,
• degrading DNA of the extracted nucleic acids with DNase,
• hybridizing mRNA of the total RNA contained in the whole single-cell with oligo (dT) fixed onto a carrier, said carrier consisting of particles and the total surface area of the particles is from 0.1 cm2 to and including 10 cm2, a volume occupancy rate of the particles in an amplification reaction solution is 1% or less, and a total number of oligo (dT) molecules fixed onto the carrier is 1012 or more,
• performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, and
• amplifying cDNA fixed onto the carrier and simultaneously detecting an amplification amount of the cDNA.