Measurement method using enzyme
AssigneesARKRAY, Inc., Kikkoman Biochemifa Company
InventorsKoji Sugiyama, Satoshi Yonehara, Kazuhiko Shimoji
Life of this patent
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Abstract
A method for measuring an analyte is described that includes the steps of: i) preparing a reagent (D) in which an enzyme (A) and an enzyme (B) coexist in the absence of the analyte; ii) bringing the analyte into contact with the enzyme (A) and the enzyme (B) so that the enzyme (A) acts on the analyte to produce a product (E), on which the enzyme (B) does not substantially act, from the analyte; iii) producing a product (C) by allowing the enzyme (A) or an enzyme (F) that is different from the enzyme (A) that acts on the analyte to produce a product (C) to act on the analyte and/or the product (E); and iv) detecting the product (C) by the enzyme (B).
Claims9
A method for measuring a glycated protein using a protease enzyme (A) that acts on the glycated protein and fructosyl amino acid oxidase (FAOD) enzyme (B) that specifically detects a glycated amino acid product (C) produced from the glycated protein, the method comprising steps of:
- i) preparing a reagent (D) in which the protease enzyme (A) and the fructosyl amino acid oxidase (FAOD) enzyme (B) coexist in the absence of the glycated protein;
- ii) bringing the glycated protein into contact with the reagent (D) to form a reaction mixture so that the protease enzyme (A) acts on the glycated protein to produce a glycated peptide product (E), on which fructosyl amino acid oxidase (FAOD) enzyme (B) does not substantially act;
- iii) producing a glycated amino acid product (C) by adding to the reaction mixture a protease enzyme (F) that is different from the protease enzyme (A) to act on the glycated peptide product (E);
- and
- iv) detecting the glycated amino acid product (C) in the reaction mixture with the fructosyl amino acid oxidase (FAOD) enzyme (B), peroxidase (POD) and a color-developing reagent to produce a detectable color.
Dependencies are inferred from the claim text.