Zeodration method for the preservation of blood platelets
AssigneesInserm (Institut National De La Sante Et De La Recherche Medicale), Universite De Strasbourg, Etablissement Francais Du Sang
InventorsFrancine Rendu, Thibault Donnet, Christian Gachet, Jean-Pierre Cazenave
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Abstract
The present invention relates to a new method for the preservation of blood platelets. In particular, the present invention provides a method which includes submitting the platelets to zeodration carried out at temperatures between 18° C. and 24° C. The dried platelets thus obtained can be preserved at room temperature for long period of time. The invention also provides kits and compositions for using the preserved platelets.
Claims15
A method for preserving blood platelets comprising steps of:
- submitting a sample of platelets to zeodration carried out between 18° C. and 24° C. inclusive to obtain dried platelets, and
- storing the dried platelets at room temperature,
- wherein zeodration comprises steps of:
- loading the sample of platelets into a chamber;
- decreasing the pressure of the chamber containing the platelets down to 700 mbar at a speed of 75 mbar/minute in less than 5 minutes;
- allowing the sample of platelets to stabilize at 700 mbar for 5 minutes;
- decreasing the pressure of the chamber containing the platelets from 700 mbar down to 200 mbar at a speed of 75 mbar/minute in less than 7 minutes;
- decreasing the pressure of the chamber containing the platelets from 200 mbar down to 145 mbar at a speed of at least 5 mbar/minute;
- and
- drying the platelets at 145 mbar for between about 2 hours and about 5 hours to obtain dried platelets having a relative humidity lower than or equal to 10% and a water activity lower than or equal to 0.6,
- wherein the steps of decreasing the pressure and of drying the platelets are performed while maintaining the temperature of the platelets between 18° C. and 24° C. inclusive.
A method for preserving blood platelets comprising steps of:
- submitting a sample of platelets to zeodration carried out between 18° C. and 24° C. inclusive to obtain dried platelets;
- and
- storing the dried platelets at room temperature;
- and
- rehydrating the dried platelets to obtain rehydrated platelets, wherein the rehydrating step comprises:
- submitting the dried platelets to a humidity gradient to obtain humidified platelets with a relative humidity of at least 65%;
- and
- adding water to the humidified platelets to obtain rehydrated platelets.
Dependencies are inferred from the claim text.