Methods and devices for separating liquid components
AssigneeCascade Medical Enterprises, LLC
InventorsNicholas A. Grippi, Roberto C. Beretta
Life of this patent
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Abstract
Methods and devices for preparing a solid-fibrin web are provided. One method may include drawing blood from a patient, separating plasma from the blood, contacting the plasma with a calcium-coagulation activator and concurrently coagulating and axially centrifuging the plasma to form the solid-fibrin web. The solid-fibrin web may be suitable for regenerating body tissue in a living organism. Devices used in the methods may also be provided.
Claims17
A device for use in axial centrifugation, the device comprising:
- a container configured for axial centrifugation, the container including
- a primary chamber configured to receive blood through a port, the primary chamber including an anticoagulant;
- a secondary chamber containing a coagulator;
- and
- a filter separating the primary chamber from the secondary chamber, the filter substantially preventing red and white blood cells, originating from the blood drawn into the primary chamber, from entering the secondary chamber under a centrifugal force of about 1000×G or greater, but substantially permitting plasma and platelets originating from the blood to flow into the secondary chamber under a centrifugal force of about 1000×G or greater;
- and
- wherein the secondary chamber optionally comprises a therapeutic enhancing agent.
A container for use in axial centrifugation, the container comprising:
- a primary chamber that can receive blood, the primary chamber including a separation medium comprising at least one of a silicone gel, polyester gel, thixotropic gel and a combination thereof;
- a secondary chamber containing a coagulator;
- and
- a diaphragm positioned between the primary chamber and the secondary chamber, the diaphragm including at least one vent initially plugged with the separation medium to prevent fluid communication between the primary chamber and the secondary chamber, the vent configured to open when the separation medium moves into the primary chamber to separate red and white blood cells from platelet rich plasma during centrifugation of at least about 1000×G to thereby allow the platelet rich plasma to flow into the secondary chamber after centrifugation;
- wherein
- the secondary chamber comprises a removable bottom, and
- wherein the primary chamber is above the secondary chamber during centrifugation.
A device for use in axial centrifugation, the device comprising:
- a container configured for axial centrifugation, the container capable of holding a fluid, the container including
- a first chamber that can receive blood, the first chamber including a separation medium comprising at least one of a silicone gel, polyester gel, thixotropic gel and a combination thereof, the first chamber having a first circumference;
- a second chamber having a second circumference and containing a coagulator, the second circumference being greater than the first circumference;
- a diaphragm positioned between the first chamber and the second chamber, the diaphragm including at least one vent initially plugged with the separation medium to prevent fluid communication between the primary chamber and the second chamber, the vent configured to open when the separation medium moves into the primary chamber to separate red and white blood cells from platelet rich plasma during centrifugation of at least about 1000×G to thereby allow the platelet rich plasma to flow into the second chamber after centrifugation;
- and wherein
- the second chamber comprises a removable bottom.
Dependencies are inferred from the claim text.