Composition and method for the restoration and preservation of transplant organs procured from DCD donors

A perfusion solution for restoring and preserving the viability of an organ recovered from a Donation After Cardiac Death (DCD) donor comprising:

• a perfusion solution, and wherein the perfusion solution further comprises:
• a metabolic support agent, wherein the metabolic support agent is present in an amount from 0.1 mM to 15 mM;
• a membrane stabilizer that is selected from the group consisting of calcium, glycine, chlorpromazine, and combinations thereof, wherein the membrane stabilizer is present in an amount from 0.1 mM to 10 mM;
• and
• an antioxidant agent selected from the group consisting of beta-carotene, catalase, superoxide dismutase, dimethyl thiourea (DMTU), diphenyl phenylene diamine (DPPD), mannitol, cyanidanol, a-tocopherol, desferoxamine, 6-hydroxy-2,5,7,8-tetramethyl chroman-2-carboxylic acid, and N-acetyl cysteine, and combinations thereof, wherein the antioxidant agent is present in an amount from 0.1 mM to 10 mM.

A perfusion solution for restoring and preserving the viability of an organ recovered from a Donation After Cardiac Death (DCD) donor comprising:

• a perfusion solution, and wherein the perfusion solution further comprises:
• a metabolic support agent that is selected from the group consisting of glucose, glutamine, and combinations thereof, and that is present in an amount from 0.1 mM to 15 mM;
• a membrane stabilizer that is selected from the group consisting of calcium, glycine, chlorpromazine, and combinations thereof and that is present in an amount from 0.1 mM to 10 mM;
• and
• an antioxidant agent selected from the group consisting of beta-carotene, catalase, superoxide dismutase, dimethyl thiourea (DMTU), diphenyl phenylene diamine (DPPD), mannitol, cyanidanol, a-tocopherol, desferoxamine, 6-hydroxy-2,5,7,8-tetramethyl chroman-2-carboxylic acid, and N-acetyl cysteine, and combinations thereof, and that is present in an amount from 0.1 mM to 10 mM.