Bile resistant Bacillus composition
AssigneeChr. Hansen A/S
InventorsInge Knap, Ane Knarreborg, Thomas Dyrmann Leser, Lund Bente, Mette Dines Cantor, Patrick Derkx
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Abstract
A bacillus composition characterized by fast germination and outgrowth in bile salts (simulated gut environment) and by producing a compound of interest. The bacillus composition may be used as supplement in animal feed where it has a probiotic (health promoting) effect and increases the digestion and availability of nutrients from animal feeds.
Claims7
A Bacillus composition comprising from 105 to 1012 CFU/g Bacillus subtilis spore cells, wherein:
- (a) the B. subtilis strain is derived from DSM 19467;
- (b) the B. subtilis spores have a germination and outgrowth from spore to vegetative cell, in the presence of a bile salt medium comprising 4 mM bile salt or in the presence of a bile salt medium comprising 6 mM bile salt, such that the B. subtilis spores reach a vegetative cell growth point of 0.4 OD630 within less than 18 hours or 19 hours;
- and
- (c) the B. subtilis vegetative cells overproduce at least one desired compound in comparison to the mother strain, DSM 19467, when grown under identical conditions.
A method for isolating a novel Bacillus subtilis cell which germinates and outgrows rapidly in the presence of a bile salt and which overproduces at least one desired compound, comprising:
- (a) subjecting vegetative cells grown from an isolated spore cell of B. subtilis strain DSM 19467 to mutagenesis, thereby obtaining a pool of individual vegetative cells which retain rapid germination and outgrowth in the presence of a bile salt;
- (b) selecting a vegetative cell from the pool of step (a), which has a germination and outgrowth from spore to vegetative cell in the presence of a bile salt medium comprising 4 mM bile salt or in the presence of a bile salt medium comprising 6 mM bile salt, such that the bacillus spores reach a vegetative cell growth point of 0.4 OD630 within less than 18 hours or 19 hours, and which overproduces said at least one desired compound in comparison to the mother strain, DSM 19467, when grown under identical conditions.
Dependencies are inferred from the claim text.