Radio-methyl vorozole and methods for making and using the same
AssigneeBrookhaven Science Associates, LLC
InventorsSung Won Kim, Anat Biegon, Joanna S. Fowler
Life of this patent
Estimating the term…
Abstract
Radiotracer vorozole compounds for in vivo and in vitro assaying, studying and imaging cytochrome P450 aromatase enzymes in humans, animals, and tissues and methods for making and using the same are provided. [N-radio-methyl] vorozole substantially separated from an N-3 radio-methyl isomer of vorozole is provided. Separation is accomplished through use of chromatography resins providing multiple mechanisms of selectivity.
Claims21
A method of preparing N-1 radio-methyl vorozole substantially separated from an N-3 radio-methyl isomer comprising:
- a) providing a mixture comprising radio-methyl vorozole and radio-methyl N-3 isomer;
- b) applying said mixture, under suitable conditions, to a chromatography resin offering multiple mechanisms for selectivity;
- c) eluting said compounds using an eluent comprising aqueous formic acid:methanol;
- thereby obtaining a composition consisting of N-1 radio-methyl vorozole.
A method of preparing N1-radio-methyl vorozole substantially separated from N2- and N3-radio-methyl isomers of vorozole comprising:
- a) providing a mixture comprising N1-radio-methyl vorozole, N2-radio-methyl isomer and N3-radio-methyl isomer;
- b) applying said mixture, under suitable conditions, to a chromatography resin selected from the group consisting of pentafluorophenylproply (PFPP) resin and phenoxypropyl resin;
- and
- c) eluting said compounds using an eluent comprising aqueous formic acid:methanol;
- thereby obtaining a composition consisting of N1-radio-methyl vorozole.
Dependencies are inferred from the claim text.