Reagent kit for sample analysis and sample analysis method

A reagent kit for analyzing a sample to measure basophils and/or nucleated erythrocytes in the sample comprising:

• a first reagent containing a cationic surfactant, a nonionic surfactant and an aromatic carboxylic acid, which can lyse erythrocytes and damage a cell membrane of leukocytes so that a fluorescent dye can permeate therethrough, and
• a second reagent containing the fluorescent dye capable of staining nucleic acid
• wherein
• said cationic surfactant is at least one selected from the group consisting of a quaternary ammonium salt and a pyridinium salt,
• said nonionic surfactant is at least one selected from the group consisting of a polyoxyethylene alkyl ether, a polyoxyethylene polyoxypropylene alkyl ether, a polyoxyethylene castor oil, a polyoxyethylene hydrogenated castor oil, a polyoxyethylene sterol and a polyoxyethylene hydrogenated sterol, and
• said fluorescent dye is at least one dye selected from the group consisting of a fluorescent dye having the general formula (I):
• wherein:
• R1 and R2 are, the same or different from each other, an alkyl group having 1 to 6 carbon atoms;
• R3, R4, R5, and R6 are, the same or different from each other, a hydrogen atom or an alkyl group;
• and X− is an anion;
• and
• a fluorescent dye having the general formula (II):
• wherein:
• R7 and R8 are, the same or different from each other, an alkyl group having 1 to 6 carbon atoms optionally containing an acidic group;
• and R9, R10, R11, and R12 are, the same or different from each other, a hydrogen atom or an acidic group, provided that either one of R7 to R12 is/has an acidic group;
• and the acidic group which may be present in R7 to R12 may form a salt, provided that either one of the acidic group(s) which may be present in R7 to R12 is a group from which a proton has been liberated.