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US 8,796,625 B2Granted
Carbon nanotube-hydrogen peroxide hybrid based optical sensing method for assessment of antioxidant potency
AssigneeBoard of Trustees of the University of Arkansas
InventorsWei Zhao, Boshan Zhao
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Abstract
An ultrasensitive dual optical method for measuring antioxidant concentration and total antioxidant capacity is provided. Using the process, the detection limit for vitamin C is as low as 7 nM. The method is based on a hybrid of single walled carbon nanotubes (SWNT) with hydrogen-peroxide. The method provides is a robust, cheap, fast, and reusable optical sensing method for antioxidant and total antioxidant capacity measurement in foods and plasma. The method can also be developed as a miniature assay for both in vitro and in vivo detection.
Claims25
A method comprising:
- measuring a first absorbance value of a composition comprising a charge transfer complex of single walled carbon nanotubes and H2O2 at one or more wavelengths in the near infra-red;
- adding a sample to the composition;
- subsequently measuring a second absorbance value of the composition at the one or more wavelengths;
- and
- determining the difference between the first and second absorbance values;
- wherein the difference between the first and second absorbance values is an indication of the antioxidant concentration or the antioxidant capacity of the sample;
- wherein the composition comprises H2O2 in excess such that some of the H2O2 molecules are part of the charge transfer complex and some of the H2O2 molecules are not part of the charge transfer complex prior to adding the sample to the composition.
4 more elements
A method comprising:
- (a) measuring a first absorbance value of a composition comprising a charge transfer complex of single walled carbon nanotubes and H2O2 at one or more wavelengths in the near infra-red;
- (b) adding a calibration sample comprising a known concentration of an antioxidant to the composition;
- (c) subsequently measuring a second absorbance value of the composition at the one or more wavelengths;
- (d) determining the difference between the first and second absorbance values;
- (e) repeating steps (a)-(d) for one or more additional calibration samples having different known concentrations of the antioxidant;
- and
- (f) determining the relationship between antioxidant concentration and the difference between the first and second absorbance values;
- wherein the composition comprises H2O2 in excess such that some of the H2O2 molecules are part of the charge transfer complex and some of the H2O2 molecules are not part of the charge transfer complex prior to adding the sample to the composition.
5 more elements
Dependencies are inferred from the claim text.