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US 8,796,440 B2Granted
Promote system for regulatable gene expression in mammalian cells
AssigneeThe Brigham and Women's Hospital, Inc.
InventorFeng Yao
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Abstract
The present invention is directed to a bidirectional human cytomegalovirus (hCMV) promoter that can be used to promote transcription on both strands of a double stranded DNA molecule. When used as part of a system that includes tet operator and the gene coding for the tet repressor, the promoter can be used to induce mammalian gene expression in a highly regulated way.
Claims10
A bidirectional promoter system derived from the hCMV major immediate-early promoter and comprising the structure X-Y-Z or X-Z, wherein:
- X is a sequence that is complementary to 29-130 consecutive nucleotides at the 3′ end of SEQ ID NO:2, and is located at the 5′ end of Y or Z but in a reverse orientation;
- Y is a nucleotide linker sequence that is not critical to promoter activity and that is 1-200 nucleotides in length;
- and
- Z comprises 500-604 contiguous nucleotides extending from the start site of transcription of the sequence shown as SEQ ID NO:2 in the 3′ to 5′ direction and is located at the 3′ end of Y or X, terminating at the start site of transcription.
1 more element
Dependencies are inferred from the claim text.