Efficient production of peptides
AssigneesThe Regents of the University of Colorado, a body corporate, AmideBio LLC
InventorsMichael H. B. Stowell, Jonathan Caruthers, Travis Nemkov, Brian Hiester, Leslie Boux, Mikhail Plam
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Abstract
The present invention relates to processes for the production of peptides, and the peptides produced accordingly. Peptides produced according to the invention may be produced more efficiently than peptides produced according to prior art processes. The production process of the invention may lead to advantages in yield, purity, and/or price. Methods of marketing peptides are also disclosed.
Claims25
A method for producing a target peptide, the method comprising:
- a) expressing a heterologous fusion peptide in a genetically modified cell, the heterologous fusion peptide comprising an affinity tag, a cleavable tag, and the target peptide, wherein the cleavable tag is tryptophan (Trp), and wherein the affinity tag or the cleavable tag is heterologous to the target peptide;
- b) solubilizing the heterologous fusion peptide;
- c) binding said heterologous fusion peptide to an affinity material via the affinity tag;
- d) cleaving said heterologous fusion peptide with N-chlorosuccinimide while bound to the affinity material to release the target peptide, thereby producing the target peptide;
- and
- e) removing the target peptide from the affinity material.
A method for producing a target peptide, the method comprising:
- a) expressing a heterologous fusion peptide in a genetically modified cell, the heterologous fusion peptide comprising an affinity tag, a cleavable tag, and the target peptide, wherein the cleavable tag is Trp, and wherein the affinity tag or the cleavable tag is heterologous to the target peptide;
- b) solubilizing the heterologous fusion peptide;
- c) binding said heterologous fusion peptide to an affinity material via the affinity tag;
- and
- d) cleaving said heterologous fusion peptide with N-chlorosuccinimide while bound to the affinity material to release the target peptide, thereby producing the target peptide.
A method for producing a target peptide, the method comprising:
- a) expressing a heterologous fusion peptide in a genetically modified cell, the heterologous fusion peptide comprising an affinity tag, a cleavable tag, and the target peptide, wherein the cleavable tag is Trp, and wherein the affinity tag or the cleavable tag is heterologous to the target peptide;
- b) solubilizing the heterologous fusion peptide;
- c) binding said heterologous fusion peptide to an affinity material via the affinity tag;
- and
- d) cleaving said heterologous fusion peptide with DMSO/sulfuric acid while bound to the affinity material to release the target peptide, thereby producing the target peptide.
Dependencies are inferred from the claim text.