Hydrophobic interaction chromatography method

A method for producing a polypeptide comprising a histidine-tag comprising the following steps:

• cultivating a prokaryotic or eukaryotic cell comprising a nucleic acid encoding a polypeptide comprising a histidine-tag,
• recovering the polypeptide comprising a histidine-tag from the cells or/and the cultivation medium,
• purifying the polypeptide comprising a histidine-tag with a hydrophobic interaction chromatography method comprising the following steps:
• applying a solution comprising the polypeptide with a histidine-tag to a hydrophobic interaction chromatography material, and
• recovering the polypeptide comprising a histidine-tag with a solution comprising imidazole or an imidazole-derivative from the hydrophobic interaction chromatography material and thereby producing a polypeptide comprising a histidine-tag.

A method for purifying a polypeptide comprising a histidine-tag comprising:

• applying a first solution to a hydrophobic interaction chromatography material,
• applying a second solution comprising the polypeptide comprising a histidine-tag to the hydrophobic interaction chromatography material,
• optionally applying a third solution to the hydrophobic interaction chromatography, and
• recovering and thereby producing or purifying the polypeptide comprising a histidine-tag with a fourth solution comprising imidazole or an imidazole-derivative,
• wherein the first solution comprises a first buffer substance, the second solution comprises a second buffer substance, and the fourth solution comprises a fourth buffer substance, wherein the fourth buffer substance is imidazole or an imidazole derivative, and wherein further the second buffer substance and the fourth buffer substance are different buffer substances.

A method for producing a polypeptide comprising a histidine-tag comprising:

• cultivating a prokaryotic or eukaryotic cell comprising a nucleic acid encoding a polypeptide comprising a histidine-tag,
• recovering the polypeptide comprising a histidine-tag from the cells or/and the cultivation medium,
• purifying the polypeptide comprising a histidine-tag with a hydrophobic interaction chromatography method comprising:
• applying a first solution to a hydrophobic interaction chromatography material,
• applying a second solution comprising the polypeptide comprising a histidine-tag to the hydrophobic interaction chromatography material,
• optionally applying a third solution to the hydrophobic interaction chromatography, and
• recovering and thereby producing or purifying the polypeptide comprising a histidine-tag with a fourth solution comprising imidazole or an imidazole-derivative,
• wherein the first solution comprises a first buffer substance, the second solution comprises a second buffer substance, and the fourth solution comprises a fourth buffer substance, wherein the fourth buffer substance is imidazole or an imidazole derivative, and wherein further the second buffer substance and the fourth buffer substance are different buffer substances.