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US 8,796,240 B2Granted
Cell growth inhibitor and screening method thereof
AssigneeThe University of Tokyo
InventorsSatoshi Inoue, Kenichi Takayama
Life of this patent
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Abstract
An object is to provide a cell growth inhibitor also effective for androgen-independent prostate cancer. The present invention provides a cell growth inhibitor having, as an active ingredient, an expression inhibitor or function inhibitor of an antisense RNA (CTBP1-AS) expressed in the vicinity of an androgen receptor (AR) binding site of a C-terminal binding protein (CTBP1) gene.
Claims10
A cell growth inhibitor
- comprising a CTBP1-AS (C-terminal binding protein 1-antisense) expression inhibitor or function inhibitor as an active ingredient, wherein
- the CTBP1-AS expression inhibitor or function inhibitor is a double-stranded RNA having an RNAi effect against CTBP1-AS and
- one strand of the double-stranded RNA comprises a base sequence complementary to the base sequence of any of SEQ ID NO:3 to 6 and 24 to 52.
A method of screening growth inhibitors of cells in which CTBP1-AS has been expressed, comprising:
- bringing cells in which CTBP1-AS has been expressed or a cell extract thereof into contact with test compounds in the presence of androgen;
- measuring an expression level of CTBP1-AS;
- and
- selecting, from the test compounds, test compounds decreasing the expression level of CTBP1-AS compared with an expression level measured in the absence of the test compounds.
1 more element
A method of screening proliferation inhibitors of cells in which CTBP1-AS has been expressed, comprising:
- bringing cells in which a CTBP1 gene has been expressed or a cell extract thereof into contact with test compounds in the presence of androgen;
- measuring an expression level of the CTBP1 gene;
- and
- selecting, from the test compounds, test compounds increasing the expression level of the CTBP1 gene compared with an expression level measured in the absence of the test compounds.
1 more element
Dependencies are inferred from the claim text.