Complex and production process
AssigneeHamlet Pharma AB
InventorsCatharina Svanborg, Kenneth Hun Mok, Ann-Kristin Mossberg, Jenny Petterson-Kastberg
Life of this patent
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Abstract
A method for preparing a biologically active complex, said method comprising a recombinant protein having the sequence of α-lactalbumin, such as human α-lactalbumin or a fragment thereof but which lacks intra-molecular disulfide bonds, and oleic acid, and process for preparing this are described and claimed. The recombinant protein suitably has cysteines found in the native protein changed to other amino acids, such as alanine. Improvements in recombinant expression, process rationalisation and yields of biologically active complexes, as well as the complexes obtained are also described and claimed.
Claims16
A method for producing a biologically active complex, said method comprising contacting a recombinant α-lactalbumin protein, which lacks intra-molecular disulfide bonds, with oleic acid under conditions in which a biologically active complex is formed, and isolating the biologically active complex, wherein cysteine residues in native α-lactalbumin have been changed to other amino acid residues in the recombinant protein.
A biologically active complex comprising a recombinant α-lactalbumin protein, which that lacks intra-molecular disulfide bonds, and oleic acid, wherein cysteine residues in native α-lactalbumin have been changed to other amino acid residues in the recombinant protein.
A method for increasing the yield of a biologically active complex comprising α-lactalbumin and oleic acid, said method comprising contacting a recombinant α-lactalbumin protein, which lacks at least some intra-molecular disulfide bonds, with oleic acid under ion exchange conditions, wherein cysteine residues in native α-lactalbumin have been changed to other amino acid residues in the recombinant protein.
A method for inhibiting the growth of a cancer cell, the method comprising contacting the cancer cell with a biologically active complex comprising a recombinant α-lactalbumin protein, which lacks intra-molecular disulfide bonds, and oleic acid, wherein cysteine residues in native α-lactalbumin have been changed to other amino acid residues in the recombinant protein.
Dependencies are inferred from the claim text.