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US 8,796,184 B2Granted
Detection assay devices and methods of making and using the same
AssigneeSentilus, Inc.
InventorsAshutosh Chilkoti, Angus Hucknall
Life of this patent
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Abstract
An article such as a biomolecular detector or biosensor having a nonfouling surface thereon includes: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; and (c) a polymer layer formed on the linking layer; and (d) a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) bound to the polymer layer. Methods of making and using the articles are also described.
Claims24
A biomolecular detector, comprising:
- (a) a substrate having a surface portion;
- (b) a linking layer on said surface portion;
- (c) a polymer layer formed on said linking layer, said polymer comprising monomeric units, with each of said monomeric units comprising a monomer core group having at least one protein-resistant head group coupled thereto, to thereby form a brush molecule on said surface portion;
- said brush molecule comprising a stem formed from the polymerization of said monomer core groups, and a plurality of branches formed from said head group projecting from said stem;
- and
- (d) a first member of a specific binding pair directly non-covalently bound to said polymer layer;
- wherein said first member is bonded to said polymer layer at a density of from 1 milligram per meter2 to 50 grams per meter2.
4 more elements
A biomolecular detector, comprising:
- (a) a substrate having a surface portion;
- (b) a linking layer on said surface portion, wherein said linking layer is patterned;
- (c) a polymer layer formed on said linking layer, said polymer comprising monomeric units, with each of said monomeric units comprising a monomer core group having at least one protein-resistant head group coupled thereto, to thereby form a brush molecule on said surface portion;
- said brush molecule comprising a stem formed from the polymerization of said monomer core groups, and a plurality of branches formed from said head group projecting from said stem;
- (d) an antibody directly non-covalently bound to said polymer layer;
- and
- (e) a plurality of additional antibodies directly non-covalently bound to said polymer layer at a plurality of additional discrete probe locations to thereby form an array thereon;
- wherein said array has a density of 10,000 to 1 million discrete probe locations per cm2 thereon.
5 more elements
A biomolecular detector, comprising:
- (a) a substrate having a surface portion;
- (b) a linking layer on said surface portion, wherein said linking layer is patterned;
- (c) a polymer layer formed on said linking layer, said polymer comprising monomeric units, with each of said monomeric units comprising a monomer core group having at least one protein-resistant head group coupled thereto, to thereby form a brush molecule on said surface portion;
- said brush molecule comprising a stem formed from the polymerization of said monomer core groups, and a plurality of branches formed from said head group projecting from said stem;
- (d) an antibody directly non-covalently bound to said polymer layer;
- and
- (e) a plurality of additional antibodies directly non-covalently bound to said polymer layer at a plurality of additional discrete probe locations to thereby form an array thereon;
- wherein said array has a density of 10,000 to 1 million discrete probe locations per cm2 thereon;
- and wherein the detector is sealed in macroscopically dry form in a container with a shelf life of at least 2 to 4 months when stored at a temperature of 25° C.
6 more elements
Dependencies are inferred from the claim text.