Method for measuring inhibitory activity on ligand-receptor binding
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorYasushi Shimonaka
Life of this patent
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Abstract
A method for measuring the presence or absence and/or strength of the inhibitory activity of a test substance on binding between a ligand and a receptor thereof, which comprises the following steps (1) to (3): (1) immobilizing either of the test substance or the ligand on a solid support; (2) contacting the test substance and the ligand for a given period of time in the presence or absence of the receptor for the ligand; and (3) comparing the level of binding between the test substance and the ligand in the presence and absence of the receptor.
Claims8
A method for detecting a test substance in a body fluid sample, comprising
- (1) immobilizing either a test substance or a ligand on a solid support;
- (2) contacting the test substance, which is contained in the body fluid sample, and the ligand for a given period of time in the presence and absence of a receptor for the ligand, wherein the test substance is an antibody against the ligand;
- (3) detecting the binding between the test substance and the ligand;
- and
- (4) comparing the level of binding between the test substance and the ligand in the presence and absence of the receptor, wherein the test substance has an inhibitory activity on binding between the ligand and the receptor if the level of binding between the test substance and the ligand in the presence of the receptor is lower than the level of binding between the test substance and the ligand in the absence of the receptor.
Dependencies are inferred from the claim text.