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AssigneeMonsanto Technology LLC
InventorsJames A. Baum, Judith C. Donovan, William P. Donovan, James T. Engleman, Karina Krasomil-Osterfeld, John W. Pitkin, James K. Roberts
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The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
A DNA construct comprising a polynucleotide operably linked to a heterologous promoter, said polynucleotide encoding a secreted Bacillus thuringiensis insecticidal protein toxic to a coleopteran insect pest, wherein said protein comprises an amino acid sequence at least 95% identical to SEQ ID NO:6 from amino acid positions 44-364.
A method for detecting a polynucleotide from a Bacillus species encoding an insecticidal protein in a sample, said protein exhibiting an amino acid sequence at least 95% identical to SEQ ID NO:6 from amino acid positions 44-364, said method comprising:
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