Methods Related to Wound Healing
AssigneeStemnion, Inc.
InventorsCharlotte A Emig, Catherine J Trumpower, Vivienne S Marshall
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Abstract
The invention is directed to methods for the treatment of wounds. Such methods utilize novel compositions, including but not limited to amnion-derived multipotent cells (herein referred to as AMP cells), conditioned media derived therefrom (herein referred to as amnion-derived cellular cytokine suspension or ACCS), cell lysates derived therefrom, cell products derived therefrom, each alone or in combination.
Claims4
A method for accelerating wound healing of a chronic, infected wound as compared to the healing rate of a non-treated chronic, infected wound in a patient in need thereof comprising treating the chronic, infected wound by topically administering to the patient's chronic, infected wound 0.5 μL-2000 μL conditioned medium per cm2 of chronic, infected wound, wherein the conditioned medium is made by
- a) obtaining a placenta and isolating an amnion from the placenta;
- b) enzymatically releasing amnion-derived epithelial cells from the amnion;
- c) collecting the released amnion-derived epithelial cells;
- d) culturing the collected amnion-derived epithelial cells of step (c) in basal culture medium that is supplemented with human serum albumin and human recombinant EGF;
- and
- e) collecting the conditioned medium.
Dependencies are inferred from the claim text.