Method for culturing neural cells using culture medium comprising carbon nanotube wires
AssigneesTsinghua University, Hon Hai Precision Industry Co., Ltd.
InventorsLi Fan, Chen Feng, Wen-Mei Zhao
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Abstract
A method for culturing neural cells using a culture medium is provided. Each neural cell includes a neural cell body and at least one neurite branched from the neural cell body. The culture medium includes a substrate and a carbon nanotube structure located on the substrate. A surface of the carbon nanotube structure is polarized to form a polar surface. The neural cells are cultured on the polar surface to grow neurites along the carbon nanotube wires. The carbon nanotube structure includes a number of carbon nanotube wires spaced apart from each other. A distance between adjacent carbon nanotube wires is greater than or equal to a diameter of the neural cell body.
Claims20
A method for culturing neural cells on a culture medium, each neural cell comprising a neural cell body and at least one neurite branched from the neural cell body, the method comprising steps of:
- providing the culture medium comprising a substrate and a carbon nanotube structure located on the substrate, the carbon nanotube structure comprising a plurality of carbon nanotube wires spaced apart from each other, wherein a distance between adjacent carbon nanotube wires is larger than or equal to a diameter of the neural cell body;
- polarizing a surface of the carbon nanotube structure to form a polar surface for providing attachment to the neural cells;
- and
- culturing the neural cells seeded directly on the polar surface to grow the neurites along the carbon nanotube wires.
A method for culturing neural cells on a culture medium, each neural cell comprising a neural cell body and at least one neurite branched from the neural cell body, the method comprising steps of:
- providing the culture medium comprising a substrate and a carbon nanotube structure located on the substrate, the carbon nanotube structure comprising a plurality of carbon nanotube wires crossed with each other to define a plurality of pores, wherein an effective diameter of each pore is greater than or equal to a diameter of the neural cell body;
- polarizing a surface of the carbon nanotube structure to form a polar surface for providing attachment to the neural cells;
- and
- culturing the neural cells seeded directly on the polar surface to grow the neurites along the carbon nanotube wires.
A method for culturing neural cells on a culture medium, each neural cell comprising a neural cell body and at least one neurite branched from the neural cell body, the method comprising steps of:
- providing the culture medium comprising a substrate and a carbon nanotube structure located on the substrate, the carbon nanotube structure comprising at least one carbon nanotube film comprising a plurality of carbon nanotube wires substantially spaced apart from each other and at least one carbon nanotube combined with adjacent carbon nanotube wires, wherein a distance between adjacent carbon nanotube wires is more than or equal to a diameter of the neural cell body;
- polarizing a surface of the carbon nanotube structure with a poly-D-lysine solution or a polyetherimide solution to form a polar surface;
- and
- culturing the neural cells seeded directly on the polar surface to grow the neurites along the carbon nanotube wires.
Dependencies are inferred from the claim text.