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US 8,796,021 B2Granted
Blastomere culture to produce mammalian embryonic stem cells
AssigneeAdvanced Cell Technology, Inc.
InventorsRobert Lanza, Young Chung
Life of this patent
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Abstract
The present invention relates generally to the field of somatic cell nuclear transfer (SCNT) and to the creation of cloned animals and cells. The disclosure relates to a method of cloning a mammal, obtaining pluripotent cells such as embryonic stem cells, or for reprogramming a mammalian cell using an oocyte and a fertilized embryo.
Claims7
A method of producing an mammalian embryonic stem (ES) cell, comprising
- (a) culturing a mammalian blastomere for 12 to 18 hours;
- (b) transferring the blastomere to blastocyst medium further comprising laminin and seeded with fibroblasts;
- and
- (c) culturing the blastomere of (b) until ES cells are produced.
1 more element
Dependencies are inferred from the claim text.