Tissue repair with multipotent cells
AssigneeCellcotec B.V.
InventorsJeanine Anna Alphonse Hendriks, Mark Ewart De Bruijn, Jens Uwe Riesle
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Abstract
The invention relates to the field of medical science, in particular to technology directed at repairing defects in living, preferably human, tissue. The present invention provides a method for inducing differentiation of multipotent cells to a desired cell type, as well as a method for repairing a tissue defect in a human or animal patient using the concept of said method for inducing differentiation of multipotent cells. The invention further relates to a kit for carrying out the method for repairing a tissue defect.
Claims31
A method for repairing a tissue defect in a human or animal patient comprising
- (a) taking a biopsy comprising a population of multipotent cells selected from the group consisting of stem cells, mesenchymal stem cells and bone marrow cells;
- (b) taking a biopsy comprising a population of primary cells selected from the group consisting of chondrocytes, cardiomyocytes and myocytes;
- (c) combining the population of multipotent cells and the population of primary cells wherein the ratio of the number of cells in the population of primary cells to the number of cells in the population of multipotent cells is from 1:200 to 1:3;
- and
- (d) applying the combined populations of multipotent cells and primary cells to the tissue defect.
A method of preparing a composition for tissue repair, comprising combining a population of multipotent cells selected from the group consisting of stem cells, mesenchymal stem cells and bone marrow cells and a population of primary cells selected from the group consisting of chondrocytes, cardiomyocytes and myocytes, wherein the ratio of the number of cells in the population of primary cells to the number of cells in the population of multipotent cells is from 1:200 to 1:3.
A method for repairing a tissue defect in a human or animal patient comprising
- (a) taking a biopsy comprising a population of multipotent cells selected from the group consisting of stem cells, mesenchymal stem cells and bone marrow cells;
- (b) taking a biopsy comprising a population of primary cells selected from the group consisting of chondrocytes, cardiomyocytes and myocytes;
- (c) combining the population of multipotent cells and the population of primary cells wherein the ratio of the number of cells in the population of primary cells to the number of cells in the population of multipotent cells is from 1:200 to 1:3;
- and
- (d) applying the combined populations of multipotent cells and primary cells to the tissue defect, wherein the primary cells are a cell type which naturally occurs in the tissue which is need of repair.
A method of preparing a composition for tissue repair, comprising combining a population of multipotent cells selected from the group consisting of stem cells, mesenchymal stem cells and bone marrow cells and a population of primary cells selected from the group consisting of chondrocytes, cardiomyocytes and myocytes, wherein the ratio of the population of primary cells to the number of multipotent cells is from 1:200 to 1:3, wherein the primary cells are a cell type which naturally occurs in the tissue which is need of repair.
Dependencies are inferred from the claim text.