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US 8,796,006 B2Granted
Crystal structure of the N-terminal domain of angiotensin converting enzyme and uses thereof
AssigneesUniversity of Bath, University of Cape Town
InventorsRavi Acharya, Edward Sturrock
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Abstract
The present invention relates to a crystal. In particular the present invention relates to a crystal of the N-domain of ACE protein. The present invention also relates to methods, processes, domain specific modulators, pharmaceutical compositions and uses of the N-domain crystal and the structure co-ordinates thereof.
Claims6
A method of preparing a crystal of the N-domain of ACE protein consisting of an amino acid sequence defined by amino acids 1-629 of SEQ ID NO:
- 8, the method comprising the steps of:
- (a) culturing host cells that express the N-domain of ACE protein;
- (b) purifying the N-domain of ACE protein;
- and
- (c) crystallising the N-domain of ACE protein using about 5 mM HEPES and about 0.1 mM phenylmethylsulfonylfluoride (PMSF) with an equal volume of a reservoir solution containing about 0.2M lithium sulphate, 15% PEG 4000, about 100 mM CH3COONa.3H2O pH 4.9 and about 10 μM ZnSO4.7H2O.
2 more elements
A method of preparing a crystal of the N-domain of ACE protein comprising:
- mixing purified recombinant N-domain of ACE protein, residues 1-629 of SEQ ID NO:
- 8, in about 5 mM HEPES and about 0.1 mM phenylmethylsulfonylfluoride (PMSF) with an equal volume of a reservoir solution containing about 0.2M lithium sulphate, 15% PEG 4000, about 100 mM CH3COONa.3H20 pH 4.9 and about 10 gM ZnSO4.7H20;
- and maintaining the mixture until a crystal grows.
Dependencies are inferred from the claim text.