Surfactant tolerant cellulase and method for modification thereof
AssigneeMeiji Seika Pharma Co., Ltd.
InventorsManabu Watanabe, Koji Yanai, Yumiko Tsuyuki
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Abstract
A method for suppressing a reduction in an endoglucanase activity in the presence of a surfactant, characterized by modifying a protein having the endoglucanase activity in which the N-terminus is an amino acid other than pyroglutamic acid, to a protein having the N-terminus of pyroglutamic acid, is disclosed. Further, a modified protein having an endoglucanase activity wherein the N-terminal amino acid is converted into pyroglutamic acid by an amino acid modification, a polynucleotide encoding the protein, an expression vector comprising the polynucleotide, a host cell transformed with the expression vector, and a process for producing the protein by cultivating the host cell, are disclosed.
Claims14
A polynucleotide encoding a protein selected from the group consisting of:
- (a) a protein comprising the amino acid sequence of SEQ ID NO:
- 2, 4, 38, or 40, wherein the N-terminal amino acid is glutamic acid or glutamine;
- and
- (b) a protein comprising an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO:
- 2, 4, 38, or 40, and having an endoglucanase activity, wherein the N-terminal amino acid is glutamic acid or glutamine.
A polynucleotide selected from the group consisting of:
- (a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
- 1, 3, 37, or 39, wherein the N-terminal amino acid is glutamic acid or glutamine;
- and
- (b) a polynucleotide hybridizing under stringent conditions to a polynucleotide consisting of the nucleotide sequence of SEQ ID NO:
- 1, 3, 37, or 39, and encoding a protein having an endoglucanase activity, wherein the N-terminal amino acid is glutamic acid or glutamine.
Dependencies are inferred from the claim text.