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US 8,795,989 B2Granted
Enzymic production of neoagarobiose
AssigneeUniversity of Maryland
InventorsSteven W. Hutcheson, Ronald M. Weiner, Nathan A. Ekborg
Life of this patent
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Abstract
The present invention is directed to methods and systems of producing neoagarobiose, useful in whitening melanoma cells and in cosmetics, using polypeptides having neoagarobiosebiohydralase activity, including Aga86E from Saccharophagus degradans. The reaction can be enhanced by including other agarases, including Aga16B, also from S. degradans.
Claims15
A method of producing neoagarobiose, comprising:
- (a) providing a substrate selected from the group consisting of agar, agarose, neoagarotetraose, agarooligosaccharides, and derivatives thereof;
- (b) contacting the substrate with a Saccharophagus degradans glycoside hydrolase (GH) polypeptide having at least 80% sequence identity with the amino acid sequence of SEQ ID NO:9, wherein the Saccharophagus degradans glycoside hydrolase (GH) polypeptide has exo-acting β-agarase activity, thereby creating a reaction mix;
- and
- (c) incubating the reaction mix under suitable conditions and sufficient time to produce neoagarobiose from the substrate.
1 more element
A method of producing neoagarobiose, comprising:
- (a) providing a substrate selected from the group consisting of agar, agarose, neoagarotetraose, agarooligosaccharides, and derivatives thereof;
- (b) contacting the substrate with a Saccharophagus degradans polypeptide having at least 90% sequence identity with the amino acid sequence of SEQ ID NO:9, wherein the Saccharophagus degradans polypeptide has exo-acting β-agarose activity, thereby creating a reaction mix;
- and
- (c) incubating the reaction mix under suitable conditions and sufficient time to produce neoagarobiose from the substrate.
1 more element
Dependencies are inferred from the claim text.