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US 8,795,967 B2Granted
Multiplex decoding of array sensors with microspheres
AssigneeIllumina, Inc.
InventorsMark S. Chee, John R. Stuelpnagel, Anthony W. Czarnik
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Abstract
The invention relates to compositions and methods for multiplex decoding of microsphere array sensors.
Claims22
A system for decoding the position of one or more bioactive agents on an array, comprising:
- an array comprising a plurality of bioactive agents randomly distributed at sites of said array, wherein each of a plurality of said sites further comprises at least a first and a second identifier binding ligand (IBL), wherein said first IBL and said second IBL each comprise different nucleic acid sequences;
- a first decoder binding ligand (DBL) adapted to bind to the first IBL, wherein binding of the first DBL with the first IBL comprises a detectable signal indicative of the location of the first IBL;
- a second DBL adapted to bind to the second IBL, wherein binding of the second DBL with the second IBL comprises a detectable signal indicative of the location of the second IBL;
- and
- wherein the location of the first and second IBLs is indicative of the position of one or more bioactive agents on said array.
2 more elements
A system for determining the presence of a target analyte in a sample, comprising:
- an array comprising a plurality of bioactive agents randomly distributed at sites of said array, wherein binding of a target analyte to said bioactive agent comprises a detectable signal;
- at least a first and a second identifier binding ligand (IBL) at each of a plurality of said sites, wherein said first IBL and said second IBL each comprise different nucleic acid sequences;
- a first decoder binding ligand (DBL) adapted to bind to the first IBL, wherein binding of the first DBL with the first IBL comprises a detectable signal indicative of the location of the first IBL;
- a second DBL adapted to bind to the second IBL, wherein binding of the second DBL with the second IBL comprises a detectable signal indicative of the location of the second IBL;
- and
- wherein the location of the first and second IBLs is indicative of the position of one or more bioactive agents on said array, wherein said first and second IBLs identify the one or more bioactive agents on said array such that the presence of an analyte can be determined.
3 more elements
Dependencies are inferred from the claim text.