Loading patent…
AssigneesEmory University, The UAB Research Foundation, Novavax, Inc.
InventorsRichard W. Compans, Baozhong Wang, Beatrice Hahn, Weimin Liu, Gale Smith, Peter Pushko
Estimating the term…
Embodiments of the present disclosure encompasses virus-like particles, methods of making virus-like particles, including expression vectors, wherein the virus-like particles may comprise enhanced levels of capsid-bound a chimeric HN-Env polypeptide compared to VLPs derived from unmodified HIV-env polypeptides. Embodiments of the virus-like particle may have Env-specific epitopes exposed on the outer surface thereof. In one embodiment, the Env-specific epitopes exposed on the outer surface of the virus-like particle may specifically bind with an anti-HIV-Env specific antibody. Embodiments of the disclosure further includes methods of generating an antibody specific to an epitope of an HIV-Env polypeptide, comprising delivering to an animal or a human an effective amount of a suspension of virus-like particles comprising a chimeric HIV-Eny polypeptide, thereby inducing the formation of an antibody specific to an epitope of an HIV-1 eny polypeptide.
A virus-like particle comprising a chimeric human immunodeficiency virus (HIV)-Env polypeptide comprising a mouse mammary tumor virus (MMTV) transmembrane domain and cytoplasmic tail.
A virus-like particle comprising a chimeric human immunodeficiency virus (HIV)-Env polypeptide wherein the virus-like particle is produced by cotransfecting a eukaryotic host cell with a first expression vector and a second expression vector, wherein the first expression vector expresses an HIV-1 gag polypeptide, and wherein the second expression vector expresses a chimeric HIV-Env polypeptide, the second expression vector comprising an expression promoter operably linked to a recombinant nucleic acid encoding, wherein the recombinant nucleic acid comprises a first domain encoding a heterologous signal peptide, wherein the first domain is operably linked to a second domain encoding an HIV-Env polypeptide region, and a third domain encoding a polypeptide region selected from the group consisting of a heterologous transmembrane region, a heterologous cytoplasmic tail region, and a combination of a heterologous transmembrane region and a heterologous cytoplasmic tail region wherein the third domain encodes a polypeptide comprising one of the amino acid sequences SEQ ID NOs.:
Dependencies are inferred from the claim text.