Back to results
US 8,790,906 B2Granted
Lysophospholipid acyltransferase
AssigneeSuntory Holdings Limited
InventorMisa Ochiai
Life of this patent
Estimating the term…
Abstract
The present invention provides novel lysophospholipid acyltransferases. The object of the present invention is attained by the nucleotide sequences of SEQ ID NOs: 1 and 6 and the amino acid sequences of SEQ ID NOs: 2 and 7 of the present invention.
Claims15
A cDNA or recombinant vector comprising a nucleic acid of any one of (a)-(e) below:
- (a) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence with deletion, substitution or addition of one to 50 amino acids in the amino acid sequence shown in SEQ ID NO:
- 7, and having lysophospholipid acyltransferase activity;
- (b) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a full length complement to the nucleotide sequence consisting of SEQ ID NO:
- 6 and that comprises a nucleotide sequence encoding a protein having lysophospholipid acyltransferase activity;
- (c) a nucleic acid that comprises a nucleotide sequence sharing an identity of 90% or more with the nucleotide sequence consisting of SEQ ID NO:
- 6 and encoding a protein having lysophospholipid acyltransferase activity;
- (d) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence sharing an identity of 90% or more with the amino acid sequence consisting of SEQ ID NO:
- 7 and having lysophospholipid acyltransferase activity;
- and
- (e) a nucleic acid that hybridizes under conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a full length complement to a nucleotide sequence encoding a protein consisting of the amino acid sequence shown in SEQ ID NO:
- 7 and that comprises a nucleotide sequence encoding a protein having lysophospholipid acyltransferase activity.
8 more elements
A cDNA or recombinant vector comprising a nucleic acid of any one of (a)-(e) below:
- (a) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence with deletion, substitution or addition of one to 50 amino acids in the amino acid sequence shown in SEQ ID NO:
- 7, and having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
- (b) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a full length complement to the nucleotide sequence consisting of SEQ ID NO:
- 6 and that comprises a nucleotide sequence encoding a protein having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
- (c) a nucleic acid that comprises a nucleotide sequence sharing an identity of 90% or more with the nucleotide sequence consisting of SEQ ID NO:
- 6 and encoding a protein having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
- (d) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence sharing an identity of 90% or more with the amino acid sequence consisting of SEQ ID NO:
- 7 and having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
- and
- (e) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a full length complement to a nucleotide sequence encoding a protein consisting of the amino acid sequence shown in SEQ ID NO:
- 7 and that comprises a nucleotide sequence encoding a protein having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing the nucleic acid as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector.
8 more elements
A cDNA or recombinant vector comprising a nucleic acid of (a) or (b) below:
- (a) a nucleic acid that comprises the nucleotide sequence shown in SEQ ID NO:
- 6;
- or
- (b) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of the amino acid sequence shown in SEQ ID NO:
- 7.
2 more elements
An isolated protein of (a) or (b) below:
- (a) a protein consisting of an amino acid sequence with deletion, substitution or addition of one to 50 amino acids in the amino acid sequence of SEQ ID NO:
- 7, and having lysophospholipid acyltransferase activity;
- or
- (b) a protein consisting of an amino acid sequence sharing an identity of 90% or more with the amino acid sequence consisting of SEQ ID NO:
- 7 and having lysophospholipid acyltransferase activity.
2 more elements
An isolated protein of (a) or (b) below:
- (a) a protein consisting of an amino acid sequence with deletion, substitution or addition of one to 50 amino acids in the amino acid sequence of SEQ ID NO:
- 7, and having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing a nucleic acid encoding the amino acid sequence as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector;
- or
- (b) a protein consisting of an amino acid sequence sharing an identity of 90% or more with the amino acid sequence consisting of SEQ ID NO:
- 7 and having the activity of increasing the proportion of arachidonic acid in the compositional ratio of fatty acids in a host transformed with a recombinant vector containing a nucleic acid encoding the amino acid sequence as compared with the proportion in the compositional ratio of fatty acids in a host that has not been transformed with the vector.
2 more elements
An isolated protein consisting of the amino acid sequence shown in SEQ ID NO:
- 7.
A cDNA or recombinant vector comprising a nucleic acid of any one of (a)-(e) below:
- (a) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence with deletion, substitution or addition of one to 50 amino acids in the amino acid sequence shown in SEQ ID NO:
- 7, and involved in the conversion from 18:3(n-6)-PL to 18:3(n-6)-CoA or conversion from DGLA-CoA to DGLA-PL;
- (b) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a full length complement to the nucleotide sequence consisting of SEQ ID NO:
- 6 and that comprises a nucleotide sequence encoding a protein involved in the conversion from 18:3(n-6)-PL to 18:3(n-6)-CoA and/or or conversion from DGLA-CoA to DGLA-PL;
- (c) a nucleic acid that comprises a nucleotide sequence sharing an identity of 90% or more with the nucleotide sequence consisting of SEQ ID NO:
- 6 and encoding a protein involved in the conversion from 18:3(n-6)-PL to 18:3(n-6)-CoA or conversion from DGLA-CoA to DGLA-PL;
- (d) a nucleic acid that comprises a nucleotide sequence encoding a protein consisting of an amino acid sequence sharing an identity of 90% or more with the amino acid sequence consisting of SEQ ID NO:
- 7 and involved in the conversion from 18:3(n-6)-PL to 18:3(n-6)-CoA or conversion from DGLA-CoA to DGLA-PL;
- and
- (e) a nucleic acid that hybridizes under hybridization conditions of 0.1×SSC-1×SSC at 60° C.-65° C. and washing conditions of 0.2×SSC-2×SSC at 50° C.-68° C. to a nucleic acid consisting of a nucleotide sequence complementary full length complement to a nucleotide sequence encoding a protein consisting of the amino acid sequence shown in SEQ ID NO:
- 7 and that comprises a nucleotide sequence encoding a protein involved in the conversion from 18:3(n-6)-PL to 18:3(n-6)-CoA or conversion from DGLA-CoA to DGLA-PL.
8 more elements
An isolated protein of (a) or (b) below:
- (a) a protein consisting of an amino acid sequence with deletion, substitution or addition of one to 50 amino acids in the amino acid sequence of SEQ ID NO:
- 7, and involved in the conversion from 18:3(n-6)-PL to 18:3(n-6)-CoA or conversion from DGLA-CoA to DGLA-PL;
- or
- (b) a protein consisting of an amino acid sequence sharing an identity of 90% or more with the amino acid sequence consisting of SEQ ID NO:
- 7 and involved in the conversion from 18:3(n-6)-PL to 18:3(n-6)-CoA or conversion from DGLA-CoA to DGLA-PL.
2 more elements
Dependencies are inferred from the claim text.