# US 8,785,848 B2 · Parallel ion mass and ion mobility analysis

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- Number: US 8,785,848 B2
- Document ID: US8785848B2
- Status: Granted
- Kind: B2
- Application number: 13/792,043
- Filed: 2013-03-09
- Published: 2013-07-25
- Granted: 2014-07-22
- Earliest claimed: 2002-03-10
- Assignees: Excellims Corporation
- Applicants: Ching Wu; Clinton Alawn Krueger; Anthony Joseph Midey; Mark A. Osgood
- Inventors: Ching Wu; Clinton Alawn Krueger; Anthony Joseph Midey; Mark A. Osgood
- Web page: https://patentgrep.com/patent/US8785848B2

## CPC

- H01J 49/009: {Spectrometers having multiple channels, parallel analysis}
- G01N 27/623: combined with mass spectrometry
- G01N 30/7206: {interfaced to gas chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04)}
- G01N 30/7233: {interfaced to liquid or supercritical fluid chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04)}
- H01J 49/0027: {Methods for using particle spectrometers}
- H01J 49/0031: {Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence)}
- H01J 49/004: {Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn}
- H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present invention relates to a parallel IMS and MS measurement method where a sample flow is split and delivered to an IMS and a MS in parallel. A parallel acquisition MS/IMS method is used to supplement LC-MS and or MS data by using a synchronized MS/IMS acquisition.

## Claims

### Claim 1

A method for identifying components of a sample in mass spectral data comprising:
a. splitting a sample flow into a mass analyzer and an ion mobility based separator for independent parallel ion mass and ion mobility analysis;
b. acquiring mass spectral data of the sample using the mass analyzer while synchronously acquiring ion mobility data using the ion mobility based separator;
c. performing a normalization between corresponding peaks of the mass spectral data and that of the peaks in the ion mobility data; and
d. displaying which ion mobility peaks correspond to the mass spectra peaks.

### Claim 2 (depends on 1)

The method in claim 1, wherein the normalization is accomplished by using a timing index and/or a calibrant.

### Claim 3 (depends on 2)

The method in claim 2, wherein the timing index is created based on the timing of each flow component's arrival at one or more analytical instrument.

### Claim 4 (depends on 3)

The method in claim 3, wherein the analytical instrument can be, but not limited to: IMS, MS, conductivity detector, UV detector, diode-array or a detector.

### Claim 5 (depends on 2)

The method in claim 2, further comprises a LC separation prior to splitting the sample flow into the mass analyzer and the ion mobility based separator.

### Claim 6 (depends on 2)

The method in claim 2, wherein the timing index is created based on the components of the sample's chromatography properties whereby the resolution obtained using the LC separation is used as a reference to determine the timing index resolution.

### Claim 7 (depends on 2)

The method in claim 2, wherein the calibrant is added to the sample flow or as one of the components of the sample.

### Claim 8 (depends on 1)

The method in claim 1, wherein the sample flow comprises isomers.

### Claim 9 (depends on 8)

The method in claim 8, wherein the isomers are constitutional isomers.

### Claim 10 (depends on 9)

The method in claim 9, wherein the constitutional isomers are skeletal isomers.

### Claim 11 (depends on 9)

The method in claim 9, wherein the constitutional isomers are regioisomers.

### Claim 12 (depends on 9)

The method in claim 9, wherein the constitutional isomers are functional group isomers.

### Claim 13 (depends on 9)

The method in claim 9, wherein the constitutional isomers are topoisomers.

### Claim 14 (depends on 8)

The method in claim 8, wherein the isomers are stereoisomers.

### Claim 15 (depends on 14)

The method in claim 14, wherein the stereoisomers are diastereomers.

### Claim 16 (depends on 14)

The method in claim 14, wherein the stereoisomers are enantiomers.

### Claim 17 (depends on 14)

The method in claim 14, wherein the stereoisomers are cis-trans isomers.

### Claim 18 (depends on 14)

The method in claim 14, wherein the stereoisomers are conformers.

### Claim 19 (depends on 14)

The method in claim 14, wherein the stereoisomers are rotamers.

### Claim 20 (depends on 14)

The method in claim 14, wherein the stereoisomers are atropisomers.