# US 8,785,730 B2 · Lettuce variety Paulita (LS9498)

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- Number: US 8,785,730 B2
- Document ID: US8785730B2
- Status: Granted
- Kind: B2
- Application number: 13/305,814
- Filed: 2011-11-29
- Published: 2012-03-22
- Granted: 2014-07-22
- Earliest claimed: 2011-11-29
- Assignees: Syngenta Participations AG
- Applicants: Jason Argyris
- Inventors: Jason Argyris
- Web page: https://patentgrep.com/patent/US8785730B2

## CPC

- A01H 6/1472: {Lactuca sativa [lettuce]}
- A01H 5/12: Leaves
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

A lettuce cultivar, designated Paulita, is disclosed. The invention relates to the seeds of lettuce cultivar Paulita, to the plants of lettuce cultivar Paulita and to methods for producing a lettuce plant by crossing the cultivar Paulita with itself or another lettuce cultivar. The invention further relates to methods for producing a lettuce plant containing in its genetic material one or more transgenes and to the transgenic lettuce plants and plant parts produced by those methods. This invention also relates to lettuce cultivars or breeding cultivars and plant parts derived from lettuce cultivar Paulita, to methods for producing other lettuce cultivars, lines or plant parts derived from lettuce cultivar Paulita and to the lettuce plants, varieties, and their parts derived from the use of those methods. The invention further relates to hybrid lettuce seeds, plants, and plant parts produced by crossing cultivar Paulita with another lettuce cultivar.

## Claims

### Claim 1

A seed of lettuce cultivar Paulita, wherein a representative sample of seed of said cultivar was deposited under ATCC Accession No. PTA-12302.

### Claim 2 (depends on 1)

A lettuce plant, or a part thereof, produced by growing the seed of claim 1.

### Claim 3 (depends on 2)

A tissue culture produced from protoplasts or cells from the plant of claim 2, wherein said cells or protoplasts are produced from a plant part selected from the group consisting of leaf, pollen, embryo, cotyledon, hypocotyl, meristematic cell root, root tip, pistil, anther, ovule, flower, shoot, stem, seed, and petiole.

### Claim 4 (depends on 3)

A lettuce plant regenerated from the tissue culture of claim 3, wherein the plant has all of the morphological and physiological characteristics of cultivar Paulita.

### Claim 5 (depends on 2)

A method for producing a lettuce seed comprising crossing two lettuce plants and harvesting the resultant lettuce seed, wherein at least one lettuce plant is the lettuce plant of claim 2.

### Claim 6 (depends on 5)

A lettuce seed produced by the method of claim 5.

### Claim 7 (depends on 6)

A lettuce plant, or a part thereof, produced by growing said seed of claim 6.

### Claim 8 (depends on 5)

The method of claim 5, wherein at least one of said lettuce plants is transgenic.

### Claim 9 (depends on 2)

A method of producing a male sterile lettuce plant, wherein the method comprises introducing a nucleic acid molecule that confers male sterility into the lettuce plant of claim 2.

### Claim 10 (depends on 9)

A male sterile lettuce plant produced by the method of claim 9.

### Claim 11 (depends on 2)

A method of producing an herbicide resistant lettuce plant, wherein said method comprises introducing a gene conferring herbicide resistance into the plant of claim 2, wherein the gene confers resistance to an herbicide selected from the group consisting of glyphosate, sulfonylurea, imidazolinone, dicamba, glufosinate, phenoxy proprionic acid, L-phosphinothricin, cyclohexone, cyclohexanedione, triazine, and benzonitrile.

### Claim 12 (depends on 11)

An herbicide resistant lettuce plant produced by the method of claim 11.

### Claim 13 (depends on 2)

A method of producing a pest or insect resistant lettuce plant, wherein said method comprises introducing a gene conferring pest or insect resistance into the plant of claim 2.

### Claim 14 (depends on 13)

A pest or insect resistant lettuce plant produced by the method of claim 13.

### Claim 15 (depends on 14)

The lettuce plant of claim 14, wherein the gene encodes a Bacillus thuringiensis endotoxin.

### Claim 16 (depends on 2)

A method of producing a disease resistant lettuce plant, wherein said method comprises introducing a gene conferring disease resistance into the plant of claim 2.

### Claim 17 (depends on 16)

A disease resistant lettuce plant produced by the method of claim 16.

### Claim 18 (depends on 2)

A method of producing a lettuce plant with a value-added trait, wherein said method comprises introducing a gene conferring a value-added trait into the plant of claim 2, where said gene encodes a protein selected from the group consisting of a ferritin, a nitrate reductase, and a monellin.

### Claim 19 (depends on 18)

A lettuce plant with a value-added trait produced by the method of claim 18.

### Claim 20

A method of introducing a desired trait into lettuce cultivar Paulita wherein the method comprises:
(a) crossing a Paulita plant, wherein a representative sample of seed was deposited under ATCC Accession No. PTA-12302, with a plant of another lettuce cultivar that comprises a desired trait to produce progeny plants wherein the desired trait is selected from the group consisting of male sterility, herbicide resistance, insect or pest resistance, modified bolting and resistance to bacterial disease, fungal disease or viral disease;
(b) selecting one or more progeny plants that have the desired trait to produce selected progeny plants;
(c) crossing the selected progeny plants with the Paulita plant to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait and all of the physiological and morphological characteristics of lettuce cultivar Paulita listed in Table 1; and
(e) repeating steps (c) and (d) two or more times in succession to produce selected third or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of lettuce cultivar Paulita listed in Table 1.

### Claim 21 (depends on 20)

A lettuce plant produced by the method of claim 20, wherein the plant has the desired trait.

### Claim 22 (depends on 21)

The lettuce plant of claim 21, wherein the desired trait is herbicide resistance and the resistance is conferred to an herbicide selected from the group consisting of glyphosate, sulfonylurea, imidazolinone, dicamba, glufosinate, phenoxy proprionic acid, L-phosphinothricin, cyclohexone, cyclohexanedione, triazine, and benzonitrile.

### Claim 23 (depends on 21)

The lettuce plant of claim 21, wherein the desired trait is insect or pest resistance and the insect or pest resistance is conferred by a transgene encoding a Bacillus thuringiensis endotoxin.