# US 8,785,729 B2 · Lettuce variety redglace

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- Number: US 8,785,729 B2
- Document ID: US8785729B2
- Status: Granted
- Kind: B2
- Application number: 13/205,971
- Filed: 2011-08-09
- Published: 2012-04-19
- Granted: 2014-07-22
- Earliest claimed: 2011-08-09
- Assignees: Nunhems B.V.
- Applicants: Jan van Schijndel
- Inventors: Jan van Schijndel
- Web page: https://patentgrep.com/patent/US8785729B2

## CPC

- A01H 6/1472: {Lactuca sativa [lettuce]}
- A01H 5/12: Leaves
- A23L 19/00: Products from fruits or vegetables; Preparation or treatment thereof (marmalades, jams, jellies or the like A23L21/10; treating harvested fruit or vegetables in bulk A23N)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)

## Abstract

The present invention relates to a lettuce variety NUN 9044 LT (referred to as “REDGLACE”) having resistance against at least strains Bl: 1 to Bl: 26 of downy mildew (Bremia lactucae) and mature leaves which exhibit red color throughout their apical part. The invention further relates to methods for producing the new lettuce variety and representative seed having been deposited under Accession No. NCIMB 42228.

## Claims

### Claim 1

A lettuce plant designated NUN 9044 LT, referred to as REDGLACE, a representative sample of seeds of which having been deposited under Accession Number NCIMB 42228.

### Claim 2

A seed designated NUN 9044 LT, a representative sample of seeds having been deposited under Accession Number NCIMB 42228.

### Claim 3 (depends on 2)

A plant, or a part thereof, produced by growing the seed of claim 2.

### Claim 4 (depends on 1)

A part of the plant of claim 1, wherein the part is a head or a leaf or a part thereof.

### Claim 5 (depends on 1)

A cell culture or tissue culture of the lettuce plant of claim 1.

### Claim 6 (depends on 1)

A lettuce plant which is regenerated from the part of the plant of claim 1 and comprises essentially all physiological and/or morphological characteristics of NUN 9044 LT.

### Claim 7 (depends on 5)

A lettuce plant which is regenerated from the cell culture or tissue culture of claim 5 and comprises essentially all physiological and/or morphological characteristics of NUN 9044 LT.

### Claim 8

A progeny plant of lettuce variety designated NUN 9044 LT obtained by further breeding with said variety, wherein said progeny plant has at least the essential physiological and/or morphological characteristic of the variety designated NUN 9044 LT, a representative sample of seeds of which having been deposited under Accession Number NCIMB 42228 wherein said progeny further has black (grey-brown) seed color.

### Claim 9 (depends on 8)

The progeny of claim 8, wherein said progeny further has at least one of the physiological and/or morphological characteristics selected from the group consisting of a length width index of fourth leaves of 10 to 20, coarsely dentate basal margin of fourth leaves (see USDA descriptors), moderately dentate apical margin of fourth leaves, a light green color at the base of mature leaves and a red color throughout at least 30% of the apical part of the mature leaves, an intense anthocyanin concentration of the at least 30% of the apical part of the mature leaves (see USDA and UPOV descriptors), slight undulation of fourth leaves (see USDA descriptors), strong undulation of the apical margin (see USDA descriptors), glossy mature leaves (see USDA descriptors) and moderate blistering of mature leaves (see USDA descriptors).

### Claim 10

An Essentially Derived Variety of NUN 9044 LT having at least one, two or three physiological and/or morphological characteristics which are different from those of NUN 9044 LT and which otherwise has essentially all physiological and/or morphological characteristics of a lettuce plant designated NUN 9044 LT, a representative sample of seeds of which having been deposited under Accession Number NCIMB 42228.

### Claim 11 (depends on 10)

An Essentially Derived Variety of NUN 9044 LT according to claim 10 having one or two physiological and/or morphological characteristics which are different from those of NUN 9044 LT and which otherwise has essentially all physiological and/or morphological characteristics of a lettuce plant designated NUN 9044 LT obtainable by selecting a natural or induced mutant, or a somaclonal variant from a population of plants designated NUN 9044 LT.

### Claim 12 (depends on 1)

A package comprising a head, leaves or parts thereof of a plant of claim 1.

### Claim 13 (depends on 2)

A package comprising the seed of claim 2.

### Claim 14 (depends on 2)

A seed pellet comprising the seed of claim 2.

### Claim 15 (depends on 1)

A method of producing a lettuce plant, comprising crossing the plant of claim 1 with a second lettuce plant one or more times and/or selfing the plant of claim 1 one or more times, and selecting progeny from said crossing and/or selfing.

### Claim 16 (depends on 1)

A method of producing a lettuce plant derived from NUN 9044 LT comprising the steps of:
(a) preparing a progeny plant derived from NUN 9044 LT by crossing the plant of claim 1 with a second lettuce plant;
(b) crossing the progeny plant with itself or a second lettuce plant to produce a seed of a progeny plant of a subsequent generation;
(c) growing a progeny plant of a subsequent generation from said seed and crossing the progeny plant of a subsequent generation with itself or a second lettuce plant; and
(d) repeating step b) or c) for at least 1 more generation to produce a lettuce plant derived from NUN 9044 LT.

### Claim 17 (depends on 1)

A method of producing a hybrid lettuce seed, comprising crossing a first parent lettuce plant with a second parent lettuce plant and harvesting the resultant hybrid lettuce seed, wherein said first parent lettuce plant or said second parent lettuce plant is a lettuce plant designated NUN 9044 LT of claim 1.

### Claim 18 (depends on 1)

A hybrid lettuce plant produced by crossing a first parent lettuce plant with a second parent lettuce plant and growing the resultant hybrid lettuce seed, wherein said first parent lettuce plant or said second parent lettuce plant is a lettuce plant designated NUN 9044 LT of claim 1.

### Claim 19

A method of introducing a single locus conversion into a lettuce plant designated NUN 9044 LT comprising
(a) crossing a plant of variety designated NUN 9044 LT with a second lettuce plant comprising a desired single locus to produce at least one F1 progeny plant;
(b) selecting F1 progeny plant that has said desired single locus;
(c) crossing the selected progeny plant NUN 9044 LT to produce backcross progeny plants;
(d) selecting backcross progeny plant that has said desired single locus and at least the essential physiological and/or morphological characteristic of a plant designated NUN 9044 LT to produce at least one selected backcross progeny plant; and
(e) repeating steps (c) and (d) one or more times in succession to produce at least one selected second or higher backcross progeny plant that comprises the single locus and otherwise comprises essentially all physiological and/or morphological characteristics of a plant designated NUN 9044 LT,
wherein a representative sample of seeds of which having been deposited under Accession Number NCIMB 42228.

### Claim 20 (depends on 19)

The method of claim 19, wherein the single locus confers a trait, wherein the trait is pest resistance or disease resistance.

### Claim 21 (depends on 19)

The method of claim 19, wherein the single locus confers at least one trait, wherein said trait is at least one trait selected from the group consisting of resistance against Nasonovia ribisnigri Nr: 1 or Nr: 0, downy mildew, Sclerotinia rot, Botrytis, powdery mildew, anthracnose, bottom rot, corky root rot, lettuce mosaic virus, big vein, lettuce aphid, beet western yellows and aster yellows, Sclerotinia minor (leaf drop), Sclerotinia sclerotiorum (leaf drop), Rhizoctonia solani (bottom drop), Erysiphe cichoracearum (powdery mildew), Fusarium oxysporum f. sp. Lactucae (fusarium wilt), lettuce infectious yellows virus (LIYV), lettuce mosaic virus (LMV), cucumber mosaic virus (CMV), beet western yellows virus (BWYV), tomato bushy stunt viru (TBSV), lettuce necrotic stunt (LNSV), Alfalfa mosaic virus (AMV), nematodes and herbicides.

### Claim 22 (depends on 1)

A plant of claim 1 wherein said plant has at least the essential physiological and/or morphological characteristics of the variety designated NUN 9044 LT and further comprises at least one transgene that confers a desired trait.