# US 8,785,728 B2 · AAD-12 event 1606 and related transgenic soybean lines

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- Number: US 8,785,728 B2
- Document ID: US8785728B2
- Status: Granted
- Kind: B2
- Application number: 13/226,789
- Filed: 2011-09-07
- Published: 2012-05-03
- Granted: 2014-07-22
- Earliest claimed: 2010-09-08
- Assignees: Dow AgroSciences LLC
- Applicants: Yunxing C. Cui; Thomas Hoffman; Ning Zhou; Gregory J. Gilles; Terry R. Wright; Dawn M. Parkhurst; Julissa Colon; Yonghe Bai
- Inventors: Yunxing C. Cui; Thomas Hoffman; Ning Zhou; Gregory J. Gilles; Terry R. Wright; Dawn M. Parkhurst; Julissa Colon; Yonghe Bai
- Web page: https://patentgrep.com/patent/US8785728B2

## CPC

- C12N 15/8274: {for herbicide resistance}
- C12N 9/0004: {Oxidoreductases (1.)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

This invention relates to soybean event pDAB4472-1606 (Event 1606). This invention includes a novel aad-12 transformation event in soybean plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a soybean cell. This invention also relates in part to plant breeding and herbicide tolerant plants. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins.

## Claims

### Claim 1

A transgenic soybean plant or cell comprising a genome, said genome comprising SEQ ID NO:1.

### Claim 2

A soybean seed comprising a genome comprising Aryloxy Alkanoate Dioxygenase-12 (AAD-12 event pDAB4472-1606 as present in the representative seed deposited with American Type Culture Collection (ATCC) under Accession No. PTA-11028.

### Claim 3 (depends on 1)

A soybean seed produced by the plant of claim 1, said seed comprising a genome, said genome comprising SEQ ID NO:1.

### Claim 4 (depends on 2)

A soybean plant produced by growing the seed of claim 2, said plant comprising SEQ ID NO:1.

### Claim 5 (depends on 4)

A progeny plant of the soybean plant of claim 4, said progeny plant comprising AAD-12 event pDAB4472-1606.

### Claim 6 (depends on 1)

A herbicide-tolerant progeny plant of the soybean plant of claim 1, said progeny plant comprising SEQ ID NO:1.

### Claim 7 (depends on 4)

A part of the plant of claim 4 wherein said part is selected from the group consisting of pollen, ovule, flowers, shoots, roots, and leaves, said part comprising SEQ ID NO:1.

### Claim 8

An isolated polynucleotide primer wherein said polynucleotide comprises a nucleotide sequence selected from the group consisting of [SEQ ID NOs: 3-26] SEQ ID NOs: 19 and 20.

### Claim 9

An isolated polynucleotide comprising SEQ ID NO:1.

### Claim 10 (depends on 1)

A method of breeding a soybean plant, said method comprising crossing a first soybean plant of claim 1 with a second soybean plant to produce a third soybean plant comprising a genome, and assaying said third soybean plant for the presence of SEQ ID NO:1 in said genome.

### Claim 11 (depends on 1)

A method of introgressing a herbicide tolerance trait into a soybean plant, said method comprising crossing the first soybean plant of claim 1 with a second soybean plant to produce a third soybean plant comprising a genome, and assaying said third soybean plant for presence of SEQ ID NO:1 in said genome.

### Claim 12 (depends on 1)

A method of controlling weeds, said method comprising applying an aryloxy alkanoate herbicide to a field, said field comprising a plant of claim 1.

### Claim 13 (depends on 12)

The method of claim 12, wherein said herbicide is 2,4-D.

### Claim 14 (depends on 12)

The method of claim 12, wherein said method comprises applying a second herbicide to said field.

### Claim 15 (depends on 14)

The method of claim 14, wherein said second herbicide is glyphosate.

### Claim 16 (depends on 3)

A method of controlling weeds, said method comprising applying an aryloxy alkanoate herbicide to a field, and planting a seed of claim 3 in said field within 14 days of applying the herbicide.