# US 8,785,726 B2 · Alteration of oil traits in plants

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- Number: US 8,785,726 B2
- Document ID: US8785726B2
- Status: Granted
- Kind: B2
- Application number: 12/429,498
- Filed: 2009-04-24
- Published: 2009-09-17
- Granted: 2014-07-22
- Earliest claimed: 2001-06-29
- Assignees: E. I. Du Pont de Nemours and Company; Pioneer Hi-Bred International, Inc.
- Applicants: William B Allen; Karlene H Butler; Rebecca E Cahoon; Sabine U Epelbaum; Changjiang Li; Igor Cunha De Oliveira; Hajime Sakai; Bo Shen; Mitchell C Tarczynski
- Inventors: William B Allen; Karlene H Butler; Rebecca E Cahoon; Sabine U Epelbaum; Changjiang Li; Igor Cunha De Oliveira; Hajime Sakai; Bo Shen; Mitchell C Tarczynski
- Web page: https://patentgrep.com/patent/US8785726B2

## CPC

- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C07K 14/415: from plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Chimeric constructs incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.

## Claims

### Claim 1

A chimeric construct comprising an isolated nucleic acid operably linked to at least one regulatory sequence, wherein the isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having an amino acid sequence of at least 95% sequence identity based on the Clustal method of alignment when compared to SEQ ID NO: 481, wherein said polypeptide is capable of altering oil phenotype in a plant, and wherein the isolated nucleic acid and the at least one regulatory sequence are not found together in nature.

### Claim 2 (depends on 1)

A plant comprising in its genome the chimeric construct of claim 1.

### Claim 3 (depends on 2)

The plant of claim 2 wherein said plant is selected from the group consisting of corn, soybean, wheat, rice, canola, Brassica, sorghum, sunflower, and coconut.

### Claim 4 (depends on 1)

A method for altering oil phenotype in a plant which comprises:
(a) transforming a plant with the chimeric construct of claim 1;
(b) growing the transformed plant under conditions suitable for expression of the chimeric construct; and
(c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.

### Claim 5 (depends on 4)

The method of claim 4 wherein the plant is selected from the group consisting of corn, soybean, wheat, rice, canola, Brassica, sorghum, sunflower, and coconut.

### Claim 6 (depends on 1)

The chimeric construct of claim 1, wherein the polypeptide has an amino acid sequence of at least 98% sequence identity based on the Clustal method of alignment when compared to SEQ ID NO: 481.

### Claim 7 (depends on 1)

The chimeric construct of claim 1, wherein the polypeptide has an amino acid sequence of at least 99% sequence identity based on the Clustal method of alignment when compared to SEQ ID NO: 481.

### Claim 8 (depends on 1)

The chimeric construct of claim 1, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 481.

### Claim 9 (depends on 1)

The chimeric construct of claim 1, wherein the nucleic acid comprises the nucleic acid sequence of SEQ ID NO: 480.

### Claim 10 (depends on 1)

A seed comprising in its genome the chimeric construct of claim 1.

### Claim 11 (depends on 10)

The seed of claim 10, wherein said seed is selected from the group consisting of corn, soybean, wheat, rice, canola, Brassica, sorghum, sunflower, and coconut.