# US 8,785,722 B2 · Dominant gene suppression transgenes and methods of using same

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- Number: US 8,785,722 B2
- Document ID: US8785722B2
- Status: Granted
- Kind: B2
- Application number: 12/907,433
- Filed: 2010-10-19
- Published: 2011-02-03
- Granted: 2014-07-22
- Earliest claimed: 2003-12-16
- Assignees: Pioneer Hi-Bred International, Inc.
- Applicants: Andrew Mark Cigan; Erica Unger
- Inventors: Andrew Mark Cigan; Erica Unger
- Web page: https://patentgrep.com/patent/US8785722B2

## CPC

- C12N 15/8241: {Phenotypically and genetically modified plants via recombinant DNA technology}
- C12N 15/8216: {Methods for controlling, regulating or enhancing expression of transgenes in plant cells}
- C12N 15/8289: {Male sterility}
- C12N 15/829: {Female sterility}
- G01N 33/5097: {involving plant cells (immunoassays of plant cells G01N33/56961)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants, and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are regulatory elements for pollen-preferred expression of linked polynucleotides. Also provided are methods for identifying gene function, methods for testing allelic variants, and methods for repressing transmission of transgenes.

## Claims

### Claim 1

A method for selectively expressing a first polynucleotide of interest in progeny of a plant, comprising:
(a) transforming said plant with a first construct comprising said polynucleotide operably linked to a first exogenous promoter, wherein said first plant further comprises a third construct comprising a pIR targeting a second exogenous promoter;
(b) transforming a second plant with a second construct comprising a promoter inverted repeat (pIR) targeting said first exogenous promoter, wherein said second plant further comprises a fourth construct comprising a second polynucleotide of interest operably linked to said second exogenous promoter;
(c) crossing said first and second plants; and
(1) selecting progeny comprising said first and second constructs, where expression of said first polynucleotide of interest is not desired; and/or
(2) selecting progeny comprising said first construct but not said second construct, where expression of said first polynucleotide of interest is desired.

### Claim 2 (depends on 1)

The method of claim 1 wherein said second or third construct comprises, or is co-transformed with, a selectable marker gene.

### Claim 3 (depends on 1)

The method of claim 1 wherein said first and second polynucleotides of interest affect the same phenotypic trait.

### Claim 4 (depends on 3)

The method of claim 3 wherein said trait is male fertility.

### Claim 5 (depends on 3)

The method of claim 3 wherein said trait is female fertility.

### Claim 6 (depends on 3)

The method of claim 3 wherein said first and second polynucleotides of interest encode the same gene product.

### Claim 7 (depends on 1)

The method of claim 1, wherein progeny are selected which comprise the fourth construct but not the third construct, where expression of the second polynucleotide of interest is desired.

### Claim 8 (depends on 1)

The method of claim 1, wherein progeny are selected which comprise the fourth construct and the third construct, where expression of the second polynucleotide of interest is not desired.