# US 8,785,720 B2 · Methods for coupling resistance alleles in tomato > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,785,720 B2 - Document ID: US8785720B2 - Status: Granted - Kind: B2 - Application number: 12/503,595 - Filed: 2009-07-15 - Published: 2010-08-19 - Granted: 2014-07-22 - Earliest claimed: 2004-02-12 - Assignees: Seminis Vegetable Seeds, Inc. - Applicants: Jacobus Gerardus Joannes Hoogstraten; Carl J. Braun, III - Inventors: Jacobus Gerardus Joannes Hoogstraten; Carl J. Braun, III - Web page: https://patentgrep.com/patent/US8785720B2 ## CPC - A01H 1/126: {for virus resistance} - A01H 5/08: Fruits - C12Q 1/6895: for plants, fungi or algae - C12Q 2600/156: Polymorphic or mutational markers - C12Q 2600/172: Haplotypes - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES ## Abstract A Lycopersicon esculentum plant comprising within its genome at least one tomato yellow leaf curl virus (TYLCV) resistance allele and at least one root knot nematode resistance allele, characterized in that the resistance alleles are present in coupling phase at different loci on one chromosome and in that the plant is highly resistant both against TYLCV and to at least one root knot nematode species selected from the group consisting of Meloidogyne arenaria, Meloidogyne incognita and Meloidogyne javanica. ## Claims ### Claim 1 A method of growing a seed from a first Lycopersicon esculentum plant comprising: a) providing said seed of said first Lycopersicon esculentum plant wherein said plant comprises within its genome at least one Ty-1 tomato yellow leaf curl virus (TYLCV) resistance allele and at least one Mi-1 root knot nematode resistance allele, wherein the at least one Ty-1 allele and the at least one Mi-1 allele are present in the coupling phase at different loci on one chromosome and said plant is resistant against TYLCV and resistant against at least one root knot nematode species selected from the group consisting of Meloidogyne arenaria, Meloidogyne incognita and Meloidogyne javanica; and b) growing said seed to provide a second Lycopersicon esculentum plant having a fruit. ### Claim 2 (depends on 1) The method of claim 1, wherein said first Lycopersicon esculentum plant has a root knot nematode resistance score of less than about 0.1. ### Claim 3 (depends on 1) The method of claim 1, wherein said first Lycopersicon esculentum plant has a root knot nematode resistance score of less than about 0.05. ### Claim 4 (depends on 1) The method of claim 1, wherein said first Lycopersicon esculentum plant has a root knot nematode resistance score of less than about 0.04. ### Claim 5 (depends on 1) The method of claim 1, wherein said first Lycopersicon esculentum plant has a root knot nematode resistance score of less than about 0.03. ### Claim 6 (depends on 1) The method of claim 1, wherein said one chromosome is chromosome 6. ### Claim 7 (depends on 1) The method according to claim 1, wherein said TYLCV resistance allele and said root knot nematode resistance allele are non-transgenic. ### Claim 8 (depends on 1) The method according to claim 1, wherein said TYLCV resistance allele and said root knot nematode resistance allele are from Lycopersicon chilense and from Lycopersicon peruvianum, respectively. ### Claim 9 (depends on 1) The method of claim 1, further comprising obtaining a second seed from said fruit, wherein said second seed comprises within its genome at least one Ty-1 yellow leaf curl virus (TYLCV) resistance allele and at least one Mi-1 root knot nematode resistance allele, wherein the at least one Ty-1 allele and the at least one Mi-1 allele are present in the coupling phase at different loci on one chromosome. ### Claim 10 (depends on 9) The method of claim 9, further comprising growing said second seed to provide a third Lycopersicon esculentum plant and crossing said third Lycopersicon esculentum plant with a fourth Lycopersicon esculentum plant. ### Claim 11 (depends on 10) A Lycopersicon esculentum plant obtained by the method of claim 10 comprising within its genome at least one tomato yellow leaf curl virus (TYLCV) resistance allele and at least one root knot nematode resistance allele, wherein said resistance alleles are present in the coupling phase at different loci on one chromosome and said plant is resistant against TYLCV and resistant against at least one root knot nematode species selected from the group consisting of Meloidogyne arenaria, Meloidogyne incognita and Meloidogyne javanica, and said at least one root knot nematode resistance allele is an allele designated as Mi-1. ### Claim 12 (depends on 11) The plant according to claim 11, wherein said plant has a root knot nematode resistance score of less than about 0.1. ### Claim 13 (depends on 11) The plant according to claim 11, wherein said TYLCV resistance allele is the allele designated as Ty-1. ### Claim 14 (depends on 9) A Lycopersicon esculentum seed obtained by the method of claim 9, comprising within its genome at least one tomato yellow leaf curl virus (TYLCV) resistance allele and at least one root knot nematode resistance allele, wherein said resistance alleles are present in the coupling phase at different loci on one chromosome and in that said plant is resistant against TYLCV and highly resistant to at least one root knot nematode species selected from Meloidogyne arenaria, Meloidogyne incognita and Meloidogyne javanica, wherein said root knot nematode resistance allele is an allele designated as Mi-1. ### Claim 15 (depends on 14) The seed according to claim 14, wherein said plant has a root knot nematode resistance score of less than about 0.1. ### Claim 16 (depends on 14) The seed according to claim 14, wherein said TYLCV resistance allele is the allele designated as Ty-1. ### Claim 17 A method of making a disease resistant L. esculentum plant comprising the steps of: a. obtaining a first parent L. esculentum plant comprising within its genome an introgression at the Ty locus from Lycopersicon chilense on chromosome 6; b. crossing said first parent L. esculentum plant with a second parent L. esculentum plant that comprises an Mi-1 introgression from Lycopersicon peruvianum on said chromosome 6; and c. selecting a progeny L. esculentum plant comprising said introgressions from L. chilense and L. peruvianum coupled in cis on chromosome 6. ### Claim 18 (depends on 17) The method of claim 17, wherein said introgression at the Ty locus from Lycopersicon chilense on chromosome 6 and said Mi-1 introgression from Lycopersicon peruvianum on said chromosome 6 overcome suppressed recombination in the Mi/Ty region of chromosome 6. ### Claim 19 A method of making a disease resistant L. esculentum plant comprising the steps of: a. obtaining a first parent L. esculentum plant comprising within its genome a Ty-1 introgression from Lycopersicon chilense on chromosome 6; b. crossing said first parent L. esculentum plant with a second parent L. esculentum plant that comprises a Mi-1 introgression from Lycopersicon peruvianum on said chromosome 6; and c. selecting a progeny L. esculentum plant comprising said Ty-1 introgression and said Mi-1 introgression coupled in cis. ### Claim 20 (depends on 1) The method of claim 1, wherein said first Lycopersicon esculentum plant is homozygous for said genome having at least one Ty-1 tomato yellow leaf curl virus (TYLCV) resistance allele and at least one Mi-1 root knot nematode resistance allele, wherein the at least one Ty-1 allele and the at least one Mi-1 allele are present in the coupling phase at different loci on one chromosome. ### Claim 21 A method of producing a Lycopersicon esculentum fruit comprising: a) planting a Lycopersicon esculentum seed wherein said seed comprises within its genome at least one Ty-1 tomato yellow leaf curl virus (TYLCV) resistance allele and at least one Mi-1 root knot nematode resistance allele, wherein the at least one Ty-1 allele and the at least one Mi-1 allele are present in the coupling phase at different loci on one chromosome; and b) growing said seed to provide a Lycopersicon esculentum plant having a fruit, wherein said Lycopersicon esculentum plant is resistant against TYLCV and resistant against at least one root knot nematode species selected from the group consisting of Meloidogyne arenaria, Meloidogyne incognita and Meloidogyne javanica. ### Claim 22 (depends on 21) The method of claim 21, wherein said one chromosome is chromosome 6. ### Claim 23 (depends on 21) The method according to claim 21, wherein said TYLCV resistance allele and said root knot nematode resistance allele are non-transgenic. ### Claim 24 (depends on 21) The method according to claim 21, wherein said TYLCV resistance allele and said root knot nematode resistance allele are from Lycopersicon chilense and from Lycopersicon peruvianum, respectively. ### Claim 25 (depends on 21) The method according to claim 21, further comprising harvesting said fruit. ### Claim 26 (depends on 21) A Lycopersicon esculentum fruit obtained by the method of claim 21, comprising within its genome at least one tomato yellow leaf curl virus (TYLCV) resistance allele and at least one root knot nematode resistance allele, wherein said resistance alleles are present in the coupling phase at different loci on one chromosome. ### Claim 27 (depends on 26) The Lycopersicon esculentum fruit according to claim 26, wherein said TYLCV resistance allele is the allele designated as Ty-1. ### Claim 28 (depends on 26) The Lycopersicon esculentum fruit according to claim 26, wherein said root knot nematode resistance allele is the allele designated as Mi-1.