# US 8,785,610 B2 · Algal desaturases

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- Number: US 8,785,610 B2
- Document ID: US8785610B2
- Status: Granted
- Kind: B2
- Application number: 13/888,310
- Filed: 2013-05-06
- Published: 2013-10-24
- Granted: 2014-07-22
- Earliest claimed: 2011-04-28
- Assignees: Aurora Algae, Inc.
- Applicants: Oliver Kilian; Bertrand Vick
- Inventors: Oliver Kilian; Bertrand Vick
- Web page: https://patentgrep.com/patent/US8785610B2

## CPC

- C12N 9/0071: {acting on paired donors with incorporation of molecular oxygen (1.14)}
- C12Y 114/19: with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

Provided herein are exemplary isolated nucleotide sequences encoding polypeptides having desaturase activity, which utilize fatty acids as substrates.

## Claims

### Claim 1

A transformation vector comprising an isolated nucleotide sequence encoding a polypeptide having desaturase activity, the nucleotide sequence set forth as SEQ ID NO:1.

### Claim 2 (depends on 1)

The transformation vector of claim 1 wherein the transformation vector encodes a functionally active desaturase which utilizes a fatty acid as a substrate.

### Claim 3 (depends on 2)

The transformation vector of claim 2, wherein the functionally active desaturase comprises amino acids having the sequence set forth as SEQ ID NO:6.

### Claim 4

A transformation vector comprising an isolated nucleotide sequence encoding a polypeptide having desaturase activity, the nucleotide sequence having at least 95% sequence identity to SEQ ID NO:2.

### Claim 5 (depends on 4)

The transformation vector sequence of claim 4 wherein the transformation vector encodes a functionally active desaturase which utilizes a fatty acid as a substrate.

### Claim 6 (depends on 5)

The transformation vector of claim 5, wherein the functionally active desaturase comprises amino acids having the sequence set forth as SEQ ID NO:7.

### Claim 7 (depends on 1)

The transformation vector of claim 1 wherein the transformation vector is derived from algae.

### Claim 8 (depends on 7)

The transformation vector of claim 7 wherein the algae is of genus Nannochloropsis.