# US 8,785,421 B1 · Use of vitamin D in dairy mastitis treatment

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- Number: US 8,785,421 B1
- Document ID: US8785421B1
- Status: Granted
- Kind: B1
- Application number: 13/434,985
- Filed: 2012-03-30
- Granted: 2014-07-22
- Earliest claimed: 2011-04-08
- Assignees: The United States of America as represented by the Secretary of Agriculture
- Applicants: John D. Lippolis; Timothy A. Reinhardt
- Inventors: John D. Lippolis; Timothy A. Reinhardt
- Web page: https://patentgrep.com/patent/US8785421B1

## CPC

- A61K 31/593: 9,10-Secocholestane derivatives, e.g. cholecalciferol, i.e. vitamin D3
- A61K 31/592: 9,10-Secoergostane derivatives, e.g. ergocalciferol, i.e. vitamin D2
- A61K 45/06: Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

Hydroxylated derivatives of vitamin D3 (cholecalciferol) and vitamin D2 (ergocalciferol) are effective for preventing or treating mastitis in animals. The hydroxylated derivatives of vitamins D3 or D2 are administered to the mammary gland of a female animal in an amount effective to inhibit or significantly reduce the growth of bacteria in the animal's mammary gland.

## Claims

### Claim 1

A method for the treatment of mastitis in animals, comprising administering a composition comprising a hydroxylated derivative of vitamin D3 or vitamin D2 or combinations thereof to the mammary gland of a female animal in need thereof, in an amount effective to inhibit the growth of bacteria in the mammary gland of said animal wherein the hydroxylated derivative is selected from the group consisting of 25-hydroxy-vitamin D3 or 1,25-dihydroxy-cholecalciferol or 25-hydroxy-vitamin D2 or 1,25-dihydroxy-vitamin D2.

### Claim 2 (depends on 1)

The method of claim 1 wherein said animal comprises a female bovine, swine, caprine or ovine.

### Claim 3 (depends on 2)

The method of claim 2 wherein said animal comprises a dairy cow.

### Claim 4 (depends on 2)

The method of claim 2 wherein said animal is lactating.

### Claim 5 (depends on 2)

The method of claim 2 wherein said animal is between lactation cycles.

### Claim 6 (depends on 1)

The method of claim 1 wherein said animal exhibits symptoms of an active mastitis infection.

### Claim 7 (depends on 1)

The method of claim 1 wherein said animal does not exhibit symptoms of an active mastitis infection.

### Claim 8 (depends on 1)

The method of claim 1 wherein said hydroxylated derivative is administered into the udder or teat of said mammary gland.

### Claim 9 (depends on 1)

The method of claim 1 wherein said hydroxylated derivative is administered by infusion of the mammary gland or using a teat cannula.

### Claim 10 (depends on 1)

The method of claim 1 wherein said hydroxylated derivative is formulated with a physiologically acceptable carrier.

### Claim 11 (depends on 10)

The method of claim 10 wherein said carrier comprises a protein.

### Claim 12 (depends on 11)

The method of claim 11 wherein said protein comprises a vitamin D binding protein.

### Claim 13 (depends on 10)

The method of claim 10 wherein said hydroxylated derivative is further formulated with an antibiotic.

### Claim 14 (depends on 1)

The method of claim 1 wherein said amount of said hydroxylated derivative is greater than about 0.05 ng/kg of body weight of said animal.

### Claim 15 (depends on 1)

The method of claim 1 wherein said amount of said hydroxylated derivative is between about 0.05 ng/kg to 2 μg/kg of body weight of said animal.

### Claim 16 (depends on 15)

The method of claim 15 wherein said amount of said hydroxylated derivative is between about 0.1 ng/kg to 1.5 μg/kg of body weight of said animal.