# US 8,785,397 B2 · Cosmetic use of apolipoprotein D type proteins

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- Number: US 8,785,397 B2
- Document ID: US8785397B2
- Status: Granted
- Kind: B2
- Application number: 12/602,246
- Filed: 2008-06-20
- Published: 2010-08-26
- Granted: 2014-07-22
- Earliest claimed: 2007-06-22
- Assignees: L'oreal
- Applicants: Dominique Bernard; Isabelle Castiel; Lucie Simonetti
- Inventors: Dominique Bernard; Isabelle Castiel; Lucie Simonetti
- Web page: https://patentgrep.com/patent/US8785397B2

## CPC

- G01N 33/92: involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones G01N33/743)}
- A61K 8/64: Proteins; Peptides; Derivatives or degradation products thereof
- A61K 38/1709: {from mammals}
- A61P 17/00: Drugs for dermatological disorders
- A61Q 17/00: Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q 19/08: Anti-ageing preparations
- C07K 14/775: Apolipopeptides
- G01N 2500/04: Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
- G01N 2500/10: involving cells
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The subject of the present invention is the use, in particular the cosmetic and/or therapeutic use, of the apolipoprotein D, of polypeptides derived from this protein or of analogues thereof, of a nucleic sequence encoding such a polypeptide or of an agent modulating the activity or of the expression of such a polypeptide, in particular for stimulating terminal epithelial differentiation. The invention also relates to the use of the protein apolipoprotein D, of polypeptides derived from this protein or of analogues thereof, or of a nucleic sequence encoding such a polypeptide as a marker for evaluating the state of the epidermis.

## Claims

### Claim 1

A method for characterizing the state of an epithelium comprising at least steps consisting in:
a) determining in a sample of the epithelium a content of a polypeptide having the amino acid sequence of SEQ ID NO:2, and
b) comparing the content determined in step a) to a reference value which is the content of the polypeptide obtained from an epithelium sample of a normal skin, the state of the epithelium being ageing, the state being characterized by a decrease between the reference value and the content determined in step a).

### Claim 2 (depends on 1)

The method according to claim 1, wherein the method is non-invasive.